Reliable, standardised, specific, and quantitative detection of genetically modified food

    Project Details


    <strong>Main research question/goal</strong><br /><div>The QPCRGMOFOOD project develops methods for the quantification of genetically modified crops in food and feed products. We focus on new strategies for method development and validation, in particular on multiplex methods and specific real-time PCR methods. An important point is also the identification of specific reference genes as an essential part for quantification of GMOs in the main food crops. The European Union as a client of this international research project. With improved waterproof detection methods, the EU aims to obtain greater confidence of consumers in the GMO legislation, and in food safety and science in general.</div><br /><strong>Research approach</strong><br /><div>This project comprises six work packages. The first involves the identification of the application and limitations of a standard DNA extraction protocol. The second work package (WP) identifies and characterises suitable species-specific reference genes, and developes reference gene-specific primer/probe sets for qualitative and quantitative PCR. WP3 involves sequence characterisation of transformation events. DNA fragments containing the junction regions are isolated and sequenced from the available reference material. In WP4, transformation event-specific primer/probe sets were developed and tested in simplex and multiplex (real-time) PCR. In WP5, the developed methods were validated in multilaboratory and collaborative trials. The final work package examined the socio-economic impact of GMO regulation and detection.</div><br /><strong>Relevance/Valorisation</strong><br /><div>This project offers information and methods that are essential for the detection of GMOs in food and feed. The criteria for acceptance of CRL (EU GMO Community Reference Laboratory) methods and the approach to the validation of methods are being established. We expect that knowledge about available and newly developed detection methods will have a long term effect on consumer confidence in food producers as well as legislators. If internationally validated and effective detection methods exist and are well-publicised, consumer confidence in the regulatory framework and food safety in general is likely to increase. The results of these activities form a solid basis for successive work of the European network of GMO laboratories (<a href=""></a>) and for EC GMO guidance documents published by the Community Reference Laboratory (<a href=""></a>).</div>
    Effective start/end date1/01/0031/12/04
    • Towards Detection of Unknown GMOs

      Holst-Jensen, A., Berdal, K., Bertheau, Y., Bohanec, M., Bohlin, J., Chaouachi, M., Gruden, K., Hamels, S., Kok, E., Krech, A., Kristoffersen, A., Laval, V., Leimanis, S., Lovoll, M., Morisset, D., Nemeth, A., Papazova, N., Prins, T., Remacle, J., Richl, P. & 8 others, Ruttink, T., Taverniers, I., Tengs, T., van Dijk, J., Wulff, D., Zel, J., Zhang, H. & Znidarsic, M., Nov-2012, Genetically modified and non-genetically modified food supply chains: co-existence and traceability. Bertheau, Y. (ed.). Wiley-Blackwell, p. 367-382 16 p.

      Research output: Chapter in Book/Report/Conference proceedingContribution to book/anthology