A general protocol for accurate gene expression analysis in plants: qPCR in plants

Ellen De Keyser; Laurence Desmet; Magali Losschaert; Jan De Riek

A general protocol for accurate gene expression analysis in plants: qPCR in plants

Ellen De Keyser, Laurence Desmet, Magali Losschaert, Jan De Riek

Plant Sciences

Research output: Chapter in Book/Report/Conference proceeding › Contribution to book/anthology › peer-review

Abstract

Gene expression analysis by means of RT-qPCR is a highly sensitive technique. However, this requires an accurate protocol for the whole procedure from sampling up to data analysis. We have optimized this protocol specifically for the analysis of plant tissues. Special attention is paid to RNA quality and integrity and to the appropriate setup of the assays in order to be compliant with the MIQE guidelines. This protocol was already successfully applied in ten different plant species.

Original language	English
Title of host publication	Quantitative Real-Time PCR : Methods and protocols
Editors	Roberto Biassoni, Alessandro Raso
Number of pages	14
Volume	2
Place of Publication	New York
Publisher	Humana Press, Springer
Publication date	2020
Pages	105-118
ISBN (Print)	978-1-4939-9832-6
ISBN (Electronic)	978-1-4939-9833-3
Publication status	Published - 2020

Cite this

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title = "A general protocol for accurate gene expression analysis in plants: qPCR in plants",

abstract = "Gene expression analysis by means of RT-qPCR is a highly sensitive technique. However, this requires an accurate protocol for the whole procedure from sampling up to data analysis. We have optimized this protocol specifically for the analysis of plant tissues. Special attention is paid to RNA quality and integrity and to the appropriate setup of the assays in order to be compliant with the MIQE guidelines. This protocol was already successfully applied in ten different plant species.",

author = "{De Keyser}, Ellen and Laurence Desmet and Magali Losschaert and {De Riek}, Jan",

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A general protocol for accurate gene expression analysis in plants : qPCR in plants. / De Keyser, Ellen ; Desmet, Laurence ; Losschaert, Magali et al.

Quantitative Real-Time PCR: Methods and protocols. ed. / Roberto Biassoni; Alessandro Raso. Vol. 2 New York : Humana Press, Springer, 2020. p. 105-118 (Methods in Molecular Biology; Vol. 2).

Research output: Chapter in Book/Report/Conference proceeding › Contribution to book/anthology › peer-review

TY - CHAP

T1 - A general protocol for accurate gene expression analysis in plants

T2 - qPCR in plants

AU - De Keyser, Ellen

AU - Desmet, Laurence

AU - Losschaert, Magali

AU - De Riek, Jan

PY - 2020

Y1 - 2020

N2 - Gene expression analysis by means of RT-qPCR is a highly sensitive technique. However, this requires an accurate protocol for the whole procedure from sampling up to data analysis. We have optimized this protocol specifically for the analysis of plant tissues. Special attention is paid to RNA quality and integrity and to the appropriate setup of the assays in order to be compliant with the MIQE guidelines. This protocol was already successfully applied in ten different plant species.

AB - Gene expression analysis by means of RT-qPCR is a highly sensitive technique. However, this requires an accurate protocol for the whole procedure from sampling up to data analysis. We have optimized this protocol specifically for the analysis of plant tissues. Special attention is paid to RNA quality and integrity and to the appropriate setup of the assays in order to be compliant with the MIQE guidelines. This protocol was already successfully applied in ten different plant species.

M3 - Contribution to book/anthology

SN - 978-1-4939-9832-6

VL - 2

T3 - Methods in Molecular Biology

SP - 105

EP - 118

BT - Quantitative Real-Time PCR

A2 - Biassoni, Roberto

A2 - Raso, Alessandro

PB - Humana Press, Springer

CY - New York

ER -

A general protocol for accurate gene expression analysis in plants: qPCR in plants

Abstract

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