A method to obtain disinfected Globodera infective juveniles directly from cysts

Kurt Heungens, D Mugniery, M VanMontagu, G Gheysen, A Niebel

    Research output: Contribution to journalA1: Web of Science-articlepeer-review


    In vitro inoculation methods are an efficient tool for the accurate study of the interaction between plants and parasitic nematodes. Obtaining disinfected nematodes is a crucial step in most of these methods. Most disinfection protocols involve surface sterilization of hatched juveniles, a procedure that usually leads to high mortality. This paper describes a new protocol for fast, inexpensive and easy disinfection of nematodes of the genus Globodera, directly from cysts. Mortality is low and varies between 10 and 40 % at the most. The surface-sterilized juveniles successfully infected in vitro-grown potato roots and reproduced on them.
    Original languageEnglish
    JournalFundamental And Applied Nematology
    Issue number1
    Pages (from-to)91-93
    Number of pages3
    Publication statusPublished - 1996

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