A study of genetic variation in Iris pseudacorus populations using amplified fragment length polymorphisms (AFLPs)

V Lamote; Isabel Roldan-Ruiz; E Coart; M De Loose; E Van Bockstaele

doi:10.1016/S0304-3770(02)00006-2

A study of genetic variation in Iris pseudacorus populations using amplified fragment length polymorphisms (AFLPs)

V Lamote
, Isabel Roldan-Ruiz
, E Coart
, M De Loose
, E Van Bockstaele

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Abstract

We used amplified fragment length polymorphisms (AFLPs) to conduct a study of the genetic diversity of Flemish populations of Iris pseudocorus in two hydrologically isolated river basins. AFLP analysis using four primer combinations was carried out on 88 different ramet samples, collected at nine different locations. A total of 148 polymorphic markers were scored. Samples from the basin of the Ijzer river were different from those of the basin of the Scheldt river, require can be explained by the geographical and hydrological isolation of both the basins. A high degree of genetic diversity was detected in the Populations, although the AFLP technique allowed us to identify three ramets belonging to one clone. The different genetic background in the basins of the rivers Ijzer and Scheldt enforce the use of local material in re-plantation projects for the conservation of natural genetic resources of yellow flag in both the basin. (C) 2002 Elsevier Science B.V. All rights reserved.

Original language	English
Journal	Aquatic Botany
Volume	73
Issue number	1
Pages (from-to)	19-31
Number of pages	13
ISSN	0304-3770
DOIs	https://doi.org/10.1016/S0304-3770(02)00006-2
Publication status	Published - 2002

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10.1016/S0304-3770(02)00006-2

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title = "A study of genetic variation in Iris pseudacorus populations using amplified fragment length polymorphisms (AFLPs)",

abstract = "We used amplified fragment length polymorphisms (AFLPs) to conduct a study of the genetic diversity of Flemish populations of Iris pseudocorus in two hydrologically isolated river basins. AFLP analysis using four primer combinations was carried out on 88 different ramet samples, collected at nine different locations. A total of 148 polymorphic markers were scored. Samples from the basin of the Ijzer river were different from those of the basin of the Scheldt river, require can be explained by the geographical and hydrological isolation of both the basins. A high degree of genetic diversity was detected in the Populations, although the AFLP technique allowed us to identify three ramets belonging to one clone. The different genetic background in the basins of the rivers Ijzer and Scheldt enforce the use of local material in re-plantation projects for the conservation of natural genetic resources of yellow flag in both the basin. (C) 2002 Elsevier Science B.V. All rights reserved.",

author = "V Lamote and Isabel Roldan-Ruiz and E Coart and \{De Loose\}, M and \{Van Bockstaele\}, E",

year = "2002",

doi = "10.1016/S0304-3770(02)00006-2",

language = "English",

volume = "73",

pages = "19--31",

journal = "Aquatic Botany",

issn = "0304-3770",

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TY - JOUR

T1 - A study of genetic variation in Iris pseudacorus populations using amplified fragment length polymorphisms (AFLPs)

AU - Lamote, V

AU - Roldan-Ruiz, Isabel

AU - Coart, E

AU - De Loose, M

AU - Van Bockstaele, E

PY - 2002

Y1 - 2002

N2 - We used amplified fragment length polymorphisms (AFLPs) to conduct a study of the genetic diversity of Flemish populations of Iris pseudocorus in two hydrologically isolated river basins. AFLP analysis using four primer combinations was carried out on 88 different ramet samples, collected at nine different locations. A total of 148 polymorphic markers were scored. Samples from the basin of the Ijzer river were different from those of the basin of the Scheldt river, require can be explained by the geographical and hydrological isolation of both the basins. A high degree of genetic diversity was detected in the Populations, although the AFLP technique allowed us to identify three ramets belonging to one clone. The different genetic background in the basins of the rivers Ijzer and Scheldt enforce the use of local material in re-plantation projects for the conservation of natural genetic resources of yellow flag in both the basin. (C) 2002 Elsevier Science B.V. All rights reserved.

AB - We used amplified fragment length polymorphisms (AFLPs) to conduct a study of the genetic diversity of Flemish populations of Iris pseudocorus in two hydrologically isolated river basins. AFLP analysis using four primer combinations was carried out on 88 different ramet samples, collected at nine different locations. A total of 148 polymorphic markers were scored. Samples from the basin of the Ijzer river were different from those of the basin of the Scheldt river, require can be explained by the geographical and hydrological isolation of both the basins. A high degree of genetic diversity was detected in the Populations, although the AFLP technique allowed us to identify three ramets belonging to one clone. The different genetic background in the basins of the rivers Ijzer and Scheldt enforce the use of local material in re-plantation projects for the conservation of natural genetic resources of yellow flag in both the basin. (C) 2002 Elsevier Science B.V. All rights reserved.

U2 - 10.1016/S0304-3770(02)00006-2

DO - 10.1016/S0304-3770(02)00006-2

M3 - A1: Web of Science-article

SN - 0304-3770

VL - 73

SP - 19

EP - 31

JO - Aquatic Botany

JF - Aquatic Botany

IS - 1

ER -

A study of genetic variation in Iris pseudacorus populations using amplified fragment length polymorphisms (AFLPs)

Abstract

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