Chromosome doubling of Cichorium intybus L. haploids through protoplast regeneration

Jeroen Van der Veken, Tom Eeckhaut, Joost Baert, O. Maudoux, C. Notté, Werbrouck Stefaan, Johan Van Huylenbroeck

Research output: Chapter in Book/Report/Conference proceedingC1: Articles in proceedings


Industrial chicory (Cichorium intybus L.) is mainly grown for inulin production. To enhance production and uniformity, hybrid cultivars are preferred. Therefore, a controlled pollination system and homozygous parental lines are needed. The most desirable pollination control system is cytoplasmic male sterility (CMS). A new, naturally induced CMS has been reported by Van der Veken et al. (2018). To regenerate homozygous parental lines of modern industrial chicory cultivars, different haploid inducing techniques have been compared and evaluated. The only successful method was intergeneric hybridization with Cicerbita alpina (Doré et al., 1996), yielding haploid plants. Here we show that protoplast regeneration is an efficient tool for chromosome doubling of these haploids. The presented method can be used for chromosome doubling of slowly developing haploids.
Original languageEnglish
Title of host publicationXXX International Horticultural Congress IHC2018: II International Symposium on Plant Breeding in Horticulture
EditorsN. Sari, Y. Aka Kacar, O. Simsek
Place of PublicationIstanbul
PublisherInternational Society for Horticultural Science
Publication date2020
ISBN (Print)978-94-62612-80-8
Publication statusPublished - 2020


Dive into the research topics of 'Chromosome doubling of <i>Cichorium intybus</i> L. haploids through protoplast regeneration'. Together they form a unique fingerprint.

Cite this