Comparative proteome analysis of porcine follicular fluid and serum reveals that excessive alpha(2)-macroglobulin in serum hampers successful expansion of cumulus-oocyte complexes

Jo Bijttebier; Kelly Tilleman; Maarten Dhaenens; Dieter Deforce; Ann Van Soom; Dominiek Maes

doi:10.1002/pmic.200900270

Comparative proteome analysis of porcine follicular fluid and serum reveals that excessive alpha(2)-macroglobulin in serum hampers successful expansion of cumulus-oocyte complexes

Jo Bijttebier
, Kelly Tilleman
, Maarten Dhaenens
, Dieter Deforce
, Ann Van Soom
, Dominiek Maes

Research output: Contribution to journal › A1: Web of Science-article

Abstract

Porcine follicular fluid (pFF) constitutes the micro-environment of the maturing oocyte. Although pFF is a transudate of serum, in pigs, it is superior to serum in promoting in vitro expansion of the cumulus cells, a specialized cell population surrounding the oocyte. A comparative proteome analysis of autologous serum and pFF was performed to investigate proteins involved in successful cumulus expansion of porcine oocytes. iTRAQ labeling followed by 2-D LC ESI-Q-TOF MS/MS revealed 63 proteins common to both fluids of which the abundance of 13 proteins was significantly different (p

Original language	English
Journal	Proteomics
Volume	9
Issue number	19
Pages (from-to)	4554-65
Number of pages	12
DOIs	https://doi.org/10.1002/pmic.200900270
Publication status	Published - 2009

Keywords

Animals
Blood Proteins
Cumulus Cells
Female
Follicular Fluid
Humans
Molecular Weight
Oocytes
Protein Array Analysis
Proteome
Swine
alpha-Macroglobulins

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10.1002/pmic.200900270

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title = "Comparative proteome analysis of porcine follicular fluid and serum reveals that excessive alpha(2)-macroglobulin in serum hampers successful expansion of cumulus-oocyte complexes",

abstract = "Porcine follicular fluid (pFF) constitutes the micro-environment of the maturing oocyte. Although pFF is a transudate of serum, in pigs, it is superior to serum in promoting in vitro expansion of the cumulus cells, a specialized cell population surrounding the oocyte. A comparative proteome analysis of autologous serum and pFF was performed to investigate proteins involved in successful cumulus expansion of porcine oocytes. iTRAQ labeling followed by 2-D LC ESI-Q-TOF MS/MS revealed 63 proteins common to both fluids of which the abundance of 13 proteins was significantly different (p",

keywords = "Animals, Blood Proteins, Cumulus Cells, Female, Follicular Fluid, Humans, Molecular Weight, Oocytes, Protein Array Analysis, Proteome, Swine, alpha-Macroglobulins",

author = "Jo Bijttebier and Kelly Tilleman and Maarten Dhaenens and Dieter Deforce and \{Van Soom\}, Ann and Dominiek Maes",

year = "2009",

doi = "10.1002/pmic.200900270",

language = "English",

volume = "9",

pages = "4554--65",

journal = "Proteomics",

issn = "1615-9861",

publisher = "Wiley-VCH Verlag",

number = "19",

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TY - JOUR

T1 - Comparative proteome analysis of porcine follicular fluid and serum reveals that excessive alpha(2)-macroglobulin in serum hampers successful expansion of cumulus-oocyte complexes

AU - Bijttebier, Jo

AU - Tilleman, Kelly

AU - Dhaenens, Maarten

AU - Deforce, Dieter

AU - Van Soom, Ann

AU - Maes, Dominiek

PY - 2009

Y1 - 2009

N2 - Porcine follicular fluid (pFF) constitutes the micro-environment of the maturing oocyte. Although pFF is a transudate of serum, in pigs, it is superior to serum in promoting in vitro expansion of the cumulus cells, a specialized cell population surrounding the oocyte. A comparative proteome analysis of autologous serum and pFF was performed to investigate proteins involved in successful cumulus expansion of porcine oocytes. iTRAQ labeling followed by 2-D LC ESI-Q-TOF MS/MS revealed 63 proteins common to both fluids of which the abundance of 13 proteins was significantly different (p

AB - Porcine follicular fluid (pFF) constitutes the micro-environment of the maturing oocyte. Although pFF is a transudate of serum, in pigs, it is superior to serum in promoting in vitro expansion of the cumulus cells, a specialized cell population surrounding the oocyte. A comparative proteome analysis of autologous serum and pFF was performed to investigate proteins involved in successful cumulus expansion of porcine oocytes. iTRAQ labeling followed by 2-D LC ESI-Q-TOF MS/MS revealed 63 proteins common to both fluids of which the abundance of 13 proteins was significantly different (p

KW - Animals

KW - Blood Proteins

KW - Cumulus Cells

KW - Female

KW - Follicular Fluid

KW - Humans

KW - Molecular Weight

KW - Oocytes

KW - Protein Array Analysis

KW - Proteome

KW - Swine

KW - alpha-Macroglobulins

U2 - 10.1002/pmic.200900270

DO - 10.1002/pmic.200900270

M3 - A1: Web of Science-article

C2 - 19688730

SN - 1615-9861

VL - 9

SP - 4554

EP - 4565

JO - Proteomics

JF - Proteomics

IS - 19

ER -

Comparative proteome analysis of porcine follicular fluid and serum reveals that excessive alpha(2)-macroglobulin in serum hampers successful expansion of cumulus-oocyte complexes

Abstract

Keywords

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