Comparative study of the discriminating capacity and effectiveness of AFLP, STMS and EST markers in assessing genetic relationships among evergreen azaleas

V. Scariot; E. De Keyser; T. Handa; J. De Riek

doi:10.1111/j.1439-0523.2007.01326.x

Comparative study of the discriminating capacity and effectiveness of AFLP, STMS and EST markers in assessing genetic relationships among evergreen azaleas

V. Scariot, E. De Keyser, T. Handa, J. De Riek

Research output: Contribution to journal › A1: Web of Science-article › peer-review

Abstract

The application of amplified fragment length polymorphism (AFLP), sequence tagged microsatellite site (STMS) and expressed sequence tag (EST) markers to study the genetic relationships in an evergreen azalea gene pool was investigated. STMS and EST markers revealed a higher genetic distance detection capacity than AFLPs, which, nevertheless, were the most efficient marker system due to their highest polymorphism detection capacity. Similarity matrices showed weak, yet significant, correlations when Mantel's test was applied. To assess the usefulness of the overall information provided by these marker data for establishing phylogenetic relationships and horticultural classification, cluster analysis was performed. The joint AFLP, STMS and EST data were demonstrated to be remarkably effective for group discrimination and phylogenetic studies. The use of these polymerase chain reaction marker systems is discussed in terms of the choice of appropriate marker techniques for different aspects of evergreen azalea germplasm evaluation

Original language	English
Journal	Plant Breeding
Volume	126
Issue number	2
Pages (from-to)	207-212
Number of pages	6
ISSN	0179-9541
DOIs	https://doi.org/10.1111/j.1439-0523.2007.01326.x
Publication status	Published - 2007

Keywords

AFLP
AGRICULTURE
amplified fragment length polymorphism
azalea
BIOTECHNOLOGY
Breeding
CAPACITY
CHAIN
CHOICE
CLASSIFICATION
cluster
cluster analysis
CLUSTER-ANALYSIS
Detection
DISCRIMINATING CAPACITY
DISCRIMINATION
ERICACEAE
EST marker
Evaluation
EVERGREEN AZALEA
EVERGREEN AZALEAS
Expressed sequence tag
expressed sequence tags
FRAGMENT LENGTH POLYMORPHISM
FRAGMENT-LENGTH-POLYMORPHISM
GENE
Genetic relationship
GENETIC-RELATIONSHIPS
Information
LENGTH
Length polymorphism
MANAGEMENT
marker comparisons
MARKERS
MICROBIOLOGY
microsatellite
MICROSATELLITES
PHYLOGENETIC-RELATIONSHIPS
phylogeny
PLANT
POLYMERASE-CHAIN-REACTION
POLYMORPHISM
REGRESSION
Rhododendron spp.
SCIENCE
SEQUENCE
sequence tagged sites
similarity
SSRS
SYSTEM
SYSTEMS
Tsutsusi

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10.1111/j.1439-0523.2007.01326.x

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title = "Comparative study of the discriminating capacity and effectiveness of AFLP, STMS and EST markers in assessing genetic relationships among evergreen azaleas",

abstract = "The application of amplified fragment length polymorphism (AFLP), sequence tagged microsatellite site (STMS) and expressed sequence tag (EST) markers to study the genetic relationships in an evergreen azalea gene pool was investigated. STMS and EST markers revealed a higher genetic distance detection capacity than AFLPs, which, nevertheless, were the most efficient marker system due to their highest polymorphism detection capacity. Similarity matrices showed weak, yet significant, correlations when Mantel's test was applied. To assess the usefulness of the overall information provided by these marker data for establishing phylogenetic relationships and horticultural classification, cluster analysis was performed. The joint AFLP, STMS and EST data were demonstrated to be remarkably effective for group discrimination and phylogenetic studies. The use of these polymerase chain reaction marker systems is discussed in terms of the choice of appropriate marker techniques for different aspects of evergreen azalea germplasm evaluation",

keywords = "AFLP, AGRICULTURE, amplified fragment length polymorphism, azalea, BIOTECHNOLOGY, Breeding, CAPACITY, CHAIN, CHOICE, CLASSIFICATION, cluster, cluster analysis, CLUSTER-ANALYSIS, Detection, DISCRIMINATING CAPACITY, DISCRIMINATION, ERICACEAE, EST marker, Evaluation, EVERGREEN AZALEA, EVERGREEN AZALEAS, Expressed sequence tag, expressed sequence tags, FRAGMENT LENGTH POLYMORPHISM, FRAGMENT-LENGTH-POLYMORPHISM, GENE, Genetic relationship, GENETIC-RELATIONSHIPS, Information, LENGTH, Length polymorphism, MANAGEMENT, marker comparisons, MARKERS, MICROBIOLOGY, microsatellite, MICROSATELLITES, PHYLOGENETIC-RELATIONSHIPS, phylogeny, PLANT, POLYMERASE-CHAIN-REACTION, POLYMORPHISM, REGRESSION, Rhododendron spp., SCIENCE, SEQUENCE, sequence tagged sites, similarity, SSRS, SYSTEM, SYSTEMS, Tsutsusi",

author = "V. Scariot and {De Keyser}, E. and T. Handa and {De Riek}, J.",

year = "2007",

doi = "10.1111/j.1439-0523.2007.01326.x",

language = "English",

volume = "126",

pages = "207--212",

journal = "Plant Breeding",

issn = "0179-9541",

publisher = "Wiley-Blackwell Publishing Ltd",

number = "2",

}

TY - JOUR

T1 - Comparative study of the discriminating capacity and effectiveness of AFLP, STMS and EST markers in assessing genetic relationships among evergreen azaleas

AU - Scariot, V.

AU - De Keyser, E.

AU - Handa, T.

AU - De Riek, J.

PY - 2007

Y1 - 2007

N2 - The application of amplified fragment length polymorphism (AFLP), sequence tagged microsatellite site (STMS) and expressed sequence tag (EST) markers to study the genetic relationships in an evergreen azalea gene pool was investigated. STMS and EST markers revealed a higher genetic distance detection capacity than AFLPs, which, nevertheless, were the most efficient marker system due to their highest polymorphism detection capacity. Similarity matrices showed weak, yet significant, correlations when Mantel's test was applied. To assess the usefulness of the overall information provided by these marker data for establishing phylogenetic relationships and horticultural classification, cluster analysis was performed. The joint AFLP, STMS and EST data were demonstrated to be remarkably effective for group discrimination and phylogenetic studies. The use of these polymerase chain reaction marker systems is discussed in terms of the choice of appropriate marker techniques for different aspects of evergreen azalea germplasm evaluation

AB - The application of amplified fragment length polymorphism (AFLP), sequence tagged microsatellite site (STMS) and expressed sequence tag (EST) markers to study the genetic relationships in an evergreen azalea gene pool was investigated. STMS and EST markers revealed a higher genetic distance detection capacity than AFLPs, which, nevertheless, were the most efficient marker system due to their highest polymorphism detection capacity. Similarity matrices showed weak, yet significant, correlations when Mantel's test was applied. To assess the usefulness of the overall information provided by these marker data for establishing phylogenetic relationships and horticultural classification, cluster analysis was performed. The joint AFLP, STMS and EST data were demonstrated to be remarkably effective for group discrimination and phylogenetic studies. The use of these polymerase chain reaction marker systems is discussed in terms of the choice of appropriate marker techniques for different aspects of evergreen azalea germplasm evaluation

KW - AFLP

KW - AGRICULTURE

KW - amplified fragment length polymorphism

KW - azalea

KW - BIOTECHNOLOGY

KW - Breeding

KW - CAPACITY

KW - CHAIN

KW - CHOICE

KW - CLASSIFICATION

KW - cluster

KW - cluster analysis

KW - CLUSTER-ANALYSIS

KW - Detection

KW - DISCRIMINATING CAPACITY

KW - DISCRIMINATION

KW - ERICACEAE

KW - EST marker

KW - Evaluation

KW - EVERGREEN AZALEA

KW - EVERGREEN AZALEAS

KW - Expressed sequence tag

KW - expressed sequence tags

KW - FRAGMENT LENGTH POLYMORPHISM

KW - FRAGMENT-LENGTH-POLYMORPHISM

KW - GENE

KW - Genetic relationship

KW - GENETIC-RELATIONSHIPS

KW - Information

KW - LENGTH

KW - Length polymorphism

KW - MANAGEMENT

KW - marker comparisons

KW - MARKERS

KW - MICROBIOLOGY

KW - microsatellite

KW - MICROSATELLITES

KW - PHYLOGENETIC-RELATIONSHIPS

KW - phylogeny

KW - PLANT

KW - POLYMERASE-CHAIN-REACTION

KW - POLYMORPHISM

KW - REGRESSION

KW - Rhododendron spp.

KW - SCIENCE

KW - SEQUENCE

KW - sequence tagged sites

KW - similarity

KW - SSRS

KW - SYSTEM

KW - SYSTEMS

KW - Tsutsusi

U2 - 10.1111/j.1439-0523.2007.01326.x

DO - 10.1111/j.1439-0523.2007.01326.x

M3 - A1: Web of Science-article

SN - 0179-9541

VL - 126

SP - 207

EP - 212

JO - Plant Breeding

JF - Plant Breeding

IS - 2

ER -

Comparative study of the discriminating capacity and effectiveness of AFLP, STMS and EST markers in assessing genetic relationships among evergreen azaleas

Abstract

Keywords

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