Derivatization and gas chromatographic-mass spectrometric detection of anabolic steroid residues isolated from edible muscle tissues

E Daeseleire, A De Guesquière, C Van Peteghem

    Research output: Contribution to journalA1: Web of Science-articlepeer-review

    Abstract

    A method was developed for the detection of anabolic steroid residues in edible muscle tissues. After enzymic digestion of the tissue and purification on disposable C18 solid-phase extraction columns, the extract was injected onto a C18 reversed-phase high-performance liquid chromatographic column. Three fractions or windows were collected, each containing specific analytes. After evaporation to dryness, the residues were subjected to a derivatization procedure which yielded suitable derivatives. After gas chromatographic-mass spectrometric analysis, both gas chromatographic retention data and mass spectral data were used for the detection and identification of nortestosterone, testosterone, estradiol, ethinylestradiol, trenbolone, methyltestosterone, chlormadinone acetate, medroxyprogesterone acetate and megestrol acetate.
    Original languageEnglish
    JournalJournal of Chromatography A
    Volume562
    Issue number1-2
    Pages (from-to)673-9
    Number of pages7
    ISSN0021-9673
    Publication statusPublished - 1991

    Keywords

    • Anabolic Agents
    • Animals
    • Cattle
    • Chromatography, High Pressure Liquid
    • Drug Residues
    • Fluorocarbons
    • Gas Chromatography-Mass Spectrometry
    • Indicators and Reagents
    • Meat
    • Muscles
    • Solvents
    • Spectrophotometry, Ultraviolet
    • Trimethylsilyl Compounds

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