Detection of murine norovirus 1 by using plaque assay, transfection assay, and real-time reverse transcription-PCR before and after heat exposure

Leen Baert; Christiane E Wobus; Els Van Coillie; Larissa B Thackray; Johan Debevere; Mieke Uyttendaele

doi:10.1128/AEM.01039-07

Detection of murine norovirus 1 by using plaque assay, transfection assay, and real-time reverse transcription-PCR before and after heat exposure

Leen Baert, Christiane E Wobus, Els Van Coillie, Larissa B Thackray, Johan Debevere, Mieke Uyttendaele

Research output: Contribution to journal › A1: Web of Science-article › peer-review

Abstract

The correlation between the detection of murine norovirus 1 RNA by real-time reverse transcription-PCR and the infectivity by plaque assay before and after heat exposure (80 degrees C) was examined. No correlation was found in the current study. Moreover, heat inactivation had a much stronger detrimental effect on virus infectivity than on the integrity of the viral genome.

Original language	English
Journal	Applied and environmental microbiology
Volume	74
Issue number	2
Pages (from-to)	543-6
Number of pages	4
ISSN	0099-2240
DOIs	https://doi.org/10.1128/AEM.01039-07
Publication status	Published - 2008

Keywords

Animals
Caliciviridae Infections
Gastroenteritis
Hot Temperature
Mice
Norovirus
RNA, Viral
Reverse Transcriptase Polymerase Chain Reaction
Temperature
Transfection
Viral Plaque Assay

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10.1128/AEM.01039-07

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title = "Detection of murine norovirus 1 by using plaque assay, transfection assay, and real-time reverse transcription-PCR before and after heat exposure",

abstract = "The correlation between the detection of murine norovirus 1 RNA by real-time reverse transcription-PCR and the infectivity by plaque assay before and after heat exposure (80 degrees C) was examined. No correlation was found in the current study. Moreover, heat inactivation had a much stronger detrimental effect on virus infectivity than on the integrity of the viral genome.",

keywords = "Animals, Caliciviridae Infections, Gastroenteritis, Hot Temperature, Mice, Norovirus, RNA, Viral, Reverse Transcriptase Polymerase Chain Reaction, Temperature, Transfection, Viral Plaque Assay",

author = "Leen Baert and Wobus, {Christiane E} and {Van Coillie}, Els and Thackray, {Larissa B} and Johan Debevere and Mieke Uyttendaele",

year = "2008",

doi = "10.1128/AEM.01039-07",

language = "English",

volume = "74",

pages = "543--6",

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TY - JOUR

T1 - Detection of murine norovirus 1 by using plaque assay, transfection assay, and real-time reverse transcription-PCR before and after heat exposure

AU - Baert, Leen

AU - Wobus, Christiane E

AU - Van Coillie, Els

AU - Thackray, Larissa B

AU - Debevere, Johan

AU - Uyttendaele, Mieke

PY - 2008

Y1 - 2008

N2 - The correlation between the detection of murine norovirus 1 RNA by real-time reverse transcription-PCR and the infectivity by plaque assay before and after heat exposure (80 degrees C) was examined. No correlation was found in the current study. Moreover, heat inactivation had a much stronger detrimental effect on virus infectivity than on the integrity of the viral genome.

AB - The correlation between the detection of murine norovirus 1 RNA by real-time reverse transcription-PCR and the infectivity by plaque assay before and after heat exposure (80 degrees C) was examined. No correlation was found in the current study. Moreover, heat inactivation had a much stronger detrimental effect on virus infectivity than on the integrity of the viral genome.

KW - Animals

KW - Caliciviridae Infections

KW - Gastroenteritis

KW - Hot Temperature

KW - Mice

KW - Norovirus

KW - RNA, Viral

KW - Reverse Transcriptase Polymerase Chain Reaction

KW - Temperature

KW - Transfection

KW - Viral Plaque Assay

U2 - 10.1128/AEM.01039-07

DO - 10.1128/AEM.01039-07

M3 - A1: Web of Science-article

C2 - 18024676

SN - 0099-2240

VL - 74

SP - 543

EP - 546

JO - Applied and Environmental Microbiology

JF - Applied and Environmental Microbiology

IS - 2

ER -

Detection of murine norovirus 1 by using plaque assay, transfection assay, and real-time reverse transcription-PCR before and after heat exposure

Abstract

Keywords

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