Determination of halofuginone in eggs by liquid chromatography/tandem mass spectrometry after cleanup with immunoaffinity chromatography

L Mortier; E Daeseleire; A-C Huet; P Delahaut; C Van Peteghem

doi:10.1002/rcm.1562

Determination of halofuginone in eggs by liquid chromatography/tandem mass spectrometry after cleanup with immunoaffinity chromatography

L Mortier, E Daeseleire, A-C Huet, P Delahaut, C Van Peteghem

Research output: Contribution to journal › A1: Web of Science-article › peer-review

Abstract

A sensitive and very selective high-performance liquid chromatography/tandem mass spectrometric (LC/MS/MS) method for the detection of halofuginone in whole egg has been developed. After deproteinisation with acetonitrile and evaporation of the organic solvent, halofuginone was further isolated by applying immunoaffinity chromatography. The concentrated eluent was injected into the LC/MS/MS system on a C18 column. The precursor ion ([M+H]+) produced by positive electrospray ionisation was selected for fragmentation with argon. Validation parameters such as recovery, linearity and repeatability, decision limit (CCalpha) and detection capability (CCbeta) were determined.

Original language	English
Journal	Rapid Communications in Mass Spectrometry
Volume	18
Issue number	16
Pages (from-to)	1817-20
Number of pages	4
ISSN	0951-4198
DOIs	https://doi.org/10.1002/rcm.1562
Publication status	Published - 2004

Keywords

Animals
Chickens
Chromatography, Affinity
Chromatography, High Pressure Liquid
Coccidiostats
Eggs
Piperidines
Quinazolines
Quinazolinones
Spectrometry, Mass, Electrospray Ionization

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10.1002/rcm.1562

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title = "Determination of halofuginone in eggs by liquid chromatography/tandem mass spectrometry after cleanup with immunoaffinity chromatography",

abstract = "A sensitive and very selective high-performance liquid chromatography/tandem mass spectrometric (LC/MS/MS) method for the detection of halofuginone in whole egg has been developed. After deproteinisation with acetonitrile and evaporation of the organic solvent, halofuginone was further isolated by applying immunoaffinity chromatography. The concentrated eluent was injected into the LC/MS/MS system on a C18 column. The precursor ion ([M+H]+) produced by positive electrospray ionisation was selected for fragmentation with argon. Validation parameters such as recovery, linearity and repeatability, decision limit (CCalpha) and detection capability (CCbeta) were determined.",

keywords = "Animals, Chickens, Chromatography, Affinity, Chromatography, High Pressure Liquid, Coccidiostats, Eggs, Piperidines, Quinazolines, Quinazolinones, Spectrometry, Mass, Electrospray Ionization",

author = "L Mortier and E Daeseleire and A-C Huet and P Delahaut and {Van Peteghem}, C",

year = "2004",

doi = "10.1002/rcm.1562",

language = "English",

volume = "18",

pages = "1817--20",

journal = "Rapid Communications in Mass Spectrometry",

issn = "0951-4198",

publisher = "John Wiley & Sons, Ltd",

number = "16",

}

TY - JOUR

T1 - Determination of halofuginone in eggs by liquid chromatography/tandem mass spectrometry after cleanup with immunoaffinity chromatography

AU - Mortier, L

AU - Daeseleire, E

AU - Huet, A-C

AU - Delahaut, P

AU - Van Peteghem, C

PY - 2004

Y1 - 2004

N2 - A sensitive and very selective high-performance liquid chromatography/tandem mass spectrometric (LC/MS/MS) method for the detection of halofuginone in whole egg has been developed. After deproteinisation with acetonitrile and evaporation of the organic solvent, halofuginone was further isolated by applying immunoaffinity chromatography. The concentrated eluent was injected into the LC/MS/MS system on a C18 column. The precursor ion ([M+H]+) produced by positive electrospray ionisation was selected for fragmentation with argon. Validation parameters such as recovery, linearity and repeatability, decision limit (CCalpha) and detection capability (CCbeta) were determined.

AB - A sensitive and very selective high-performance liquid chromatography/tandem mass spectrometric (LC/MS/MS) method for the detection of halofuginone in whole egg has been developed. After deproteinisation with acetonitrile and evaporation of the organic solvent, halofuginone was further isolated by applying immunoaffinity chromatography. The concentrated eluent was injected into the LC/MS/MS system on a C18 column. The precursor ion ([M+H]+) produced by positive electrospray ionisation was selected for fragmentation with argon. Validation parameters such as recovery, linearity and repeatability, decision limit (CCalpha) and detection capability (CCbeta) were determined.

KW - Animals

KW - Chickens

KW - Chromatography, Affinity

KW - Chromatography, High Pressure Liquid

KW - Coccidiostats

KW - Eggs

KW - Piperidines

KW - Quinazolines

KW - Quinazolinones

KW - Spectrometry, Mass, Electrospray Ionization

U2 - 10.1002/rcm.1562

DO - 10.1002/rcm.1562

M3 - A1: Web of Science-article

C2 - 15329875

SN - 0951-4198

VL - 18

SP - 1817

EP - 1820

JO - Rapid Communications in Mass Spectrometry

JF - Rapid Communications in Mass Spectrometry

IS - 16

ER -

Determination of halofuginone in eggs by liquid chromatography/tandem mass spectrometry after cleanup with immunoaffinity chromatography

Abstract

Keywords

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