Projects per year
Abstract
Boar taint is an off-odour occurring while heating meat or fat from boars. A method detecting the three compounds (androstenone, skatole and indole) simultaneously in blood would offer substantial advantages since it would allow monitoring the impact of rearing strategies. Therefore, a UHPLC-HR-Orbitrap-MS analysis method is optimized and validated for the quantification of these compounds in plasma or serum. Sample pre-treatment involved an extraction with diethylether followed by a centrifugal filtration (30 kDa). Limits of detection and quantification varied between 0.5 and 1 μg L(-1) and 2 and 3 μg L(-1) for the three compounds, respectively. Besides, an excellent repeatability (RSD < 7.6%), within-laboratory reproducibility (RSD<10.5%), recovery (87-97%) and linearity (R(2)>0.99) were recorded. Correlations between serum/plasma and fat levels of the boar taint compounds were positive for skatole (r(serum) = 0.39 and r(plasma) = 0.84) and androstenone (r(serum) = 0.73-0.78 and r(plasma) = 0.32-0.80).
Original language | English |
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Journal | FOOD CHEMISTRY |
Volume | 187 |
Pages (from-to) | 120-129 |
ISSN | 0308-8146 |
Publication status | Published - 2015 |
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Dive into the research topics of 'Development of a quantitative method for the simultaneous analysis of the boar taint compounds androstenone, skatole and indole in porcine serum and plasma by means of ultra-high performance liquid chromatography coupled to high resolution mass spectrometry'. Together they form a unique fingerprint.Projects
- 5 Finished
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BOARVAL: Valorisation of carcasses with boar taint in fresh and process meat products
Aluwé, M. (ProjectSupervisor) & De Campeneere, S. (Project Manager)
1/01/14 → 31/03/16
Project: Research
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TAINTLESS: Farm specific strategies to reduce boar taint
De Campeneere, S. (Project Manager), Aluwé, M. (ProjectSupervisor), Millet, S. (ProjectSupervisor), Tuyttens, F. (ProjectSupervisor) & Heyrman, E. (Former Researcher)
1/10/13 → 31/12/17
Project: Research
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VLEVACINIM: How to raise entire male pigs and immunocastrates
Aluwé, M. (ProjectSupervisor), De Campeneere, S. (Project Manager) & Millet, S. (ProjectSupervisor)
1/07/13 → 30/06/15
Project: Research