Development of an optimal culture system for callogenesis of Chrysanthemum indicum protoplasts

T. Eeckhaut; J. Van Huylenbroeck

doi:10.1007/s11738-010-0660-1

Development of an optimal culture system for callogenesis of Chrysanthemum indicum protoplasts

Research output: Contribution to journal › A1: Web of Science-article › peer-review

Abstract

This study is a comparison of four methods to induce calli formation in a protoplast culture of Chrysanthemum indicum. Culture in liquid medium (17.6 calli/10(5) protoplasts) was preferable to culture in solid agarose beads, although the efficiency of the latter could be improved by layering them on glass beads (12.5 vs. 0.83 calli/10(5) protoplasts). Culture of protoplasts on moistened filter paper was unsuccessful. In the liquid media, microcalli and calli were induced efficiently and easily after 6 weeks. These effects may be explained by reduced toxicity due to cell breakdown and improved aeration

Original language	English
Journal	Acta Physiologiae Plantarum
Volume	33
Issue number	4
Pages (from-to)	1547-1551
Number of pages	5
ISSN	0137-5881
DOIs	https://doi.org/10.1007/s11738-010-0660-1
Publication status	Published - 2011

Keywords

Aeration
Agarose beads
Belgium
BIOTECHNOLOGY
Breeding
CULTURE
EFFICIENCY
GERMANY
GROWTH
In vitro
Liquid medium
methods
Microcalli
PLANT
PLANT-SYSTEM
Regeneration
SCIENCE
SYSTEM

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10.1007/s11738-010-0660-1

PROTOPLASTCHRYSANT: Asymmetric protoplast fusion in Chrysanthemum
Eeckhaut, T. (Researcher) & Van Huylenbroeck, J. (Project Manager)
1/04/07 → …
Project: Research

Cite this

@article{e64e9d5754a54b899405eb2a56a97147,

title = "Development of an optimal culture system for callogenesis of Chrysanthemum indicum protoplasts",

abstract = "This study is a comparison of four methods to induce calli formation in a protoplast culture of Chrysanthemum indicum. Culture in liquid medium (17.6 calli/10(5) protoplasts) was preferable to culture in solid agarose beads, although the efficiency of the latter could be improved by layering them on glass beads (12.5 vs. 0.83 calli/10(5) protoplasts). Culture of protoplasts on moistened filter paper was unsuccessful. In the liquid media, microcalli and calli were induced efficiently and easily after 6 weeks. These effects may be explained by reduced toxicity due to cell breakdown and improved aeration",

keywords = "Aeration, Agarose beads, Belgium, BIOTECHNOLOGY, Breeding, CULTURE, EFFICIENCY, GERMANY, GROWTH, In vitro, Liquid medium, methods, Microcalli, PLANT, PLANT-SYSTEM, Regeneration, SCIENCE, SYSTEM",

author = "T. Eeckhaut and \{Van Huylenbroeck\}, J.",

note = "J English Article Eeckhaut, T (reprint author), Inst Agr \& Fisheries Res ILVO, Plant Sci Unit Appl Genet \& Breeding, Caritasstr 21, B-9090 Melle, Belgium [email protected] 16 0 SPRINGER HEIDELBERG HEIDELBERG TIERGARTENSTRASSE 17, D-69121 HEIDELBERG, GERMANY ACTA PHYSIOL PLANT JUL Discipline: Plant Sciences 777FB",

year = "2011",

doi = "10.1007/s11738-010-0660-1",

language = "English",

volume = "33",

pages = "1547--1551",

journal = "Acta Physiologiae Plantarum",

issn = "0137-5881",

publisher = "Springer Heidelberg",

number = "4",

}

TY - JOUR

T1 - Development of an optimal culture system for callogenesis of Chrysanthemum indicum protoplasts

AU - Eeckhaut, T.

AU - Van Huylenbroeck, J.

N1 - J English Article Eeckhaut, T (reprint author), Inst Agr & Fisheries Res ILVO, Plant Sci Unit Appl Genet & Breeding, Caritasstr 21, B-9090 Melle, Belgium [email protected] 16 0 SPRINGER HEIDELBERG HEIDELBERG TIERGARTENSTRASSE 17, D-69121 HEIDELBERG, GERMANY ACTA PHYSIOL PLANT JUL Discipline: Plant Sciences 777FB

PY - 2011

Y1 - 2011

N2 - This study is a comparison of four methods to induce calli formation in a protoplast culture of Chrysanthemum indicum. Culture in liquid medium (17.6 calli/10(5) protoplasts) was preferable to culture in solid agarose beads, although the efficiency of the latter could be improved by layering them on glass beads (12.5 vs. 0.83 calli/10(5) protoplasts). Culture of protoplasts on moistened filter paper was unsuccessful. In the liquid media, microcalli and calli were induced efficiently and easily after 6 weeks. These effects may be explained by reduced toxicity due to cell breakdown and improved aeration

AB - This study is a comparison of four methods to induce calli formation in a protoplast culture of Chrysanthemum indicum. Culture in liquid medium (17.6 calli/10(5) protoplasts) was preferable to culture in solid agarose beads, although the efficiency of the latter could be improved by layering them on glass beads (12.5 vs. 0.83 calli/10(5) protoplasts). Culture of protoplasts on moistened filter paper was unsuccessful. In the liquid media, microcalli and calli were induced efficiently and easily after 6 weeks. These effects may be explained by reduced toxicity due to cell breakdown and improved aeration

KW - Aeration

KW - Agarose beads

KW - Belgium

KW - BIOTECHNOLOGY

KW - Breeding

KW - CULTURE

KW - EFFICIENCY

KW - GERMANY

KW - GROWTH

KW - In vitro

KW - Liquid medium

KW - methods

KW - Microcalli

KW - PLANT

KW - PLANT-SYSTEM

KW - Regeneration

KW - SCIENCE

KW - SYSTEM

U2 - 10.1007/s11738-010-0660-1

DO - 10.1007/s11738-010-0660-1

M3 - A1: Web of Science-article

SN - 0137-5881

VL - 33

SP - 1547

EP - 1551

JO - Acta Physiologiae Plantarum

JF - Acta Physiologiae Plantarum

IS - 4

ER -

Development of an optimal culture system for callogenesis of Chrysanthemum indicum protoplasts

Abstract

Keywords

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PROTOPLASTCHRYSANT: Asymmetric protoplast fusion in Chrysanthemum

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