Discovery based high resolution MS/MS analysis for selection of allergen markers in chocolate and broth powder matrices

Rosa Pilolli, Christof Van Poucke, Elisabetta De Angelis, Chiara Nitride, Marc de Loose, Nathalie Gillard, Anne Catherine Huet, Olivier Tranquet, Colette Larré, Karine Adel-Patient, Hervé Bernard, E. N.Clare Mills, Linda Monaci

Research output: Contribution to journalArticlepeer-review


Peptide marker identification is an important step in development of a mass spectrometry method for multiple allergen detection, since specificity, robustness and sensitivity of the overall analytical method will depend on the reliability of the proteotypic peptides. As part of the development of a multi-analyte reference method, discovery analysis of two incurred food matrices has been undertaken to select the most reliable peptide markers. Six allergenic ingredients (milk, egg, peanut, soybean, hazelnut, and almond) were incurred into either chocolate or broth powder matrix. Different conditions of protein extraction and purification were tested and the tryptic peptide pools were analysed by untargeted high resolution tandem mass spectrometry and the resulting fragmentation spectra were processed via a commercial software for sequence identification. The analysis performed on incurred foods provides both a prototype effective and straightforward sample preparation protocol and delivers reliable peptides to be included in a standardized selected reaction monitoring method.
Original languageEnglish
Publication statusPublished - 2020


  • Discovery analysis
  • Food allergens
  • Incurred matrix
  • Mass spectrometry
  • Peptide markers
  • ThRAll

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