Arcobacter population dynamics in pigs on farrow-to-finish farms

Sarah De Smet, Lieven De Zutter, Lies Debruyne, Frédéric Vangroenweghe, Peter Vandamme, Kurt Houf

    Research output: Contribution to journalA1: Web of Science-articlepeer-review

    Abstract

    Healthy pigs are an important reservoir for the emerging human pathogen Arcobacter which can result in contamination of porcine carcasses and pork and the spread of arcobacters into the environment. Up to now, the excretion of arcobacters by pigs has been studied, but information about the transmission routes in fattening pigs is lacking. The present study aimed to elucidate the Arcobacter population dynamics in pigs during the fattening period on four farrow-to-finish farms. On each farm, 30 clinically healthy, 12-week-old piglets were selected. Fecal samples were collected on 10 sampling occasions until a slaughter age of 30 weeks was reached. Arcobacter spp. were isolated by a selective method and identified by multiplex PCR. The genetic diversity was examined by amplified fragment length polymorphism and enterobacterial repetitive intergenic consensus PCR. The Arcobacter presence in the fecal samples on the four farms ranged from 11.3 to 50.0%, with excretion levels of up to 10(4) CFU/g feces. The ratio in which Arcobacter species were isolated varied between the farms and over time. Characterization revealed a high degree of genotypic diversity among the isolates. Arcobacter strains persisted and spread within the finishing unit during the fattening period. The occurrence of both unique and shared genotypes in pigs in adjacent and nonadjacent pens demonstrates that transmission routes other than fecal-oral transmission occur.
    Original languageEnglish
    JournalApplied and environmental microbiology
    Volume77
    Issue number5
    Pages (from-to)1732-8
    Number of pages7
    ISSN0099-2240
    DOIs
    Publication statusPublished - 2011

    Keywords

    • Animals
    • Arcobacter
    • Bacterial Typing Techniques
    • Carrier State
    • Feces
    • Genetic Variation
    • Gram-Negative Bacterial Infections
    • Molecular Typing
    • Polymorphism, Restriction Fragment Length
    • Swine

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