Evaluation of methods measuring the capsid integrity and/or functions of noroviruses by heat inactivation

Dan Li, Leen Baert, Ming Xia, Weiming Zhong, Els Van Coillie, Xi Jiang, Mieke Uyttendaele

    Research output: Contribution to journalA1: Web of Science-articlepeer-review

    Abstract

    Attempts were made to evaluate methods measuring the capsid integrity and/or functions of noroviruses (NoVs) following heat treatment. Intact viruses (Murine Norovirus-1 [MNV-1] and human NoV GII.4), virus like particles (VLPs) and P particles (expressed in vitro from the protruding domain of the viral capsid) of NoVs were used in this study. Following heat treatment, no significant difference of viral titer of MNV-1 versus NoV GII.4 was observed by RNase One RT-PCR or cell-binding RT-PCR, although cell-binding RT-PCR (to measure the capsid functions) revealed higher reductions than RNase One RT-PCR (to measure the capsid integrity). These results indicate that the function assay for receptor binding is more sensitive than the capsid integrity assay to measure the protected viral RNA. MNV-1 could be used as a surrogate for human NoVs by heat inactivation from the perspective of capsid integrity and/or functions. The heat resistance varied among different GI and GII NoV strains when their P particles were studied.
    Original languageEnglish
    JournalJournal of virological methods
    Volume181
    Issue number1
    Pages (from-to)1-5
    Number of pages5
    DOIs
    Publication statusPublished - 2012

    Keywords

    • Hot Temperature
    • Humans
    • Microbial Viability
    • Norovirus
    • Nucleocapsid
    • Reverse Transcriptase Polymerase Chain Reaction
    • Viral Load
    • Virus Inactivation

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