Expression, purification and characterization of full-length RNA-free hepatitis B core particles

Katleen Broos, Peter Vanlandschoot, Marleen Maras, Johan Robbens, Geert Leroux-Roels, Yves Guisez

    Research output: Contribution to journalA1: Web of Science-article

    Abstract

    The nucleocapsid or core particle of the hepatitis B virus has become one of the favourite recombinant vaccine carriers for foreign peptides, proteins and stimulatory oligonucleotides. The core protein consists of three regions: an N-terminal, a central and a C-terminal region that can accommodate the addition or insertion of the foreign sequences. The protamine-like C-terminal region that binds host RNA randomly during recombinant particle formation is often truncated. It is commonly thought that these truncations do not affect particle assembly. Recent studies have demonstrated that the C-terminal domains mediate a glycosaminoglycan-dependent attachment of nucleocapsids to the plasma membranes of host cells. This interaction might well contribute to the immunogenicity of nucleocapsids. Testing the hypothesis that full-length particles might be safer and superior for the induction of an immune response against the nucleocapsids and inserted sequences, requires the availability of purified particles. In this report, we detail a novel method for the synthesis and purification of full-length core particles essentially free of RNA from Escherichia coli.
    Original languageEnglish
    JournalProtein Expression and Purification
    Volume54
    Issue number1
    Pages (from-to)30-7
    Number of pages8
    ISSN1046-5928
    DOIs
    Publication statusPublished - 2007

    Keywords

    • Biotechnology
    • Escherichia coli
    • Hepatitis B virus
    • Nucleocapsid
    • RNA, Bacterial

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