Genetic characterization of strains named as Xanthomonas axonopodis pv. dieffenbachiae leads to a taxonomic revision of the X. axonopodis species complex

Elena Constantin, Ilse Cleenwerck, Martine Maes, Steve Baeyen, Cinzia Van Malderghem, Paul De Vos, Bart Cottyn

Research output: Contribution to journalA1: Web of Science-articlepeer-review

Abstract

Xanthomonas axonopodis pv. dieffenbachiae (Xad) is the causal agent of anthurium bacterial blight and listed as an A2 quarantine organism by EPPO. However, the name Xad covers a variety of strains. Here, 25 Xad strains and 88
phylogenetically related strains, including Xanthomonas type strains and representatives of other pathovars, were examined using a polyphasic taxonomic approach. Multilocus sequence analysis of seven genes showed that strains isolated from Dieffenbachia, Philodendron and Anthurium cluster into three phylogenetic groups (PG I, II and III), while the type strain of X. axonopodis clustered into a fourth group (PG IV). PG I included the type strains of X. citri subsp. citri, X. citri subsp. malvacearum, X. fuscans subsp. fuscans and X. fuscans subsp. aurantifolii. PG II included the type strains of X. euvesicatoria, X. perforans, X. alfalfae subsp. alfalfae and X. alfalfae subsp. citrumelonis. PG III included the type strains of X. phaseoli. Each PG was shown to represent a single species, based on average nucleotide identity values, DNA–DNA hybridization data and phenotypic characteristics. Therefore, strains named as Xad belong to PG I, PG II and PG III, and not to X. axonopodis (PG IV). Taxonomic proposals are made: emendations of the descriptions of X. citri, X. phaseoli and X. axonopodis, to encompass the strains of PG I, PG III and PG IV, respectively; and reclassification of X. perforans and X. alfalfae as X. euvesicatoria and emendation of the description of X. euvesicatoria to encompass all strains of PG II.
Original languageEnglish
Article numberDoi:10.1111/ppa.12461
JournalPlant Pathology
Volume65
Pages (from-to)792-806
Number of pages15
ISSN1365-3059
DOIs
Publication statusPublished - 1-May-2016

Fingerprint

Dive into the research topics of 'Genetic characterization of strains named as Xanthomonas axonopodis pv. dieffenbachiae leads to a taxonomic revision of the X. axonopodis species complex'. Together they form a unique fingerprint.

Cite this