Hormonal and Genetic Regulation of Axillary Bud Outgrowth in Chrysanthemum morifolium during Floral Initiation

Robrecht Dierck; Ellen De Keyser; Jan De Riek; Emmy Dhooghe; Johan Van Huylenbroeck; Els Prinsen; Dominique Van Der Straeten

Hormonal and Genetic Regulation of Axillary Bud Outgrowth in Chrysanthemum morifolium during Floral Initiation

Robrecht Dierck, Ellen De Keyser, Jan De Riek, Emmy Dhooghe, Johan Van Huylenbroeck, Els Prinsen, Dominique Van Der Straeten

Research output: Chapter in Book/Report/Conference proceeding › C1: Articles in proceedings › peer-review

Abstract

Shoot branching or the outgrowth of axillary buds is a crucial aspect in the production and breeding of Chrysanthemum sp. The outgrowth of axillary buds is inhibited by the shoot apex through apical dominance. Central to this regulation is the interaction of the plant hormones auxin, cytokinin and strigolactone. In this research we aim to study the hormonal and genetic regulation of apical dominance and axillary bud outgrowth in a cut flower cultivar of Chrysanthemum. For this we combined measurements of axillary bud outgrowth with UPLC-MS/MS quantification of auxin and cytokinin levels and RT-qPCR analysis of two genes involved in the regulation of branching. The cultivar used displayed apical dominance during vegetative growth. A release from apical dominance occurs during floral initiation with outgrowth and elongation of shoots under the apex. The outgrowth of axillary buds after release from apical dominance was preceded by decreased auxin levels in the shoot apex, axillary buds and stem and increased cytokinin levels in the axillary buds. RT-qPCR showed a decreased expression of the local bud outgrowth regulator BRC1 preceding the release from apical dominance at the time of floral initiation.

Original language	English
Title of host publication	Proceedings of the XXVth International Eucarpia Symposium Section Ornamentals "Crossing Borders"
Editors	Johan Van Huylenbroeck, Emmy Dhooghe
Number of pages	8
Volume	1087
Publication date	2015
Pages	179-185
ISBN (Print)	9789462610781
Publication status	Published - 2015
Event	25th International Eucarpia Symposium Section Ornamentals: Crossing Borders - Melle, Belgium Duration: 28-Jun-2015 → 2-Jul-2015

IWT-STURING SIERTEELT: Knowledge-driven regulation of plant physiological processes in ornamentals to improve plant quality
De Keyser, E., Van Huylenbroeck, J., Cnops, G., De Riek, J., Lootens, P., Dierck, R. & Dhooghe, E.
1/10/12 → 30/09/18
Project: Research

25th International Eucarpia Symposium Section Ornamentals: Crossing Borders
Emmy Dhooghe (Co-organisator)
28-Jun-2015 → 2-Jul-2015
Activity: Participating in or organising an event › Organisation and participation in conference

Cite this

Dierck, R., De Keyser, E., De Riek, J., Dhooghe, E., Van Huylenbroeck, J., Prinsen, E., & Van Der Straeten , D. (2015). Hormonal and Genetic Regulation of Axillary Bud Outgrowth in Chrysanthemum morifolium during Floral Initiation. In J. Van Huylenbroeck, & E. Dhooghe (Eds.), Proceedings of the XXVth International Eucarpia Symposium Section Ornamentals "Crossing Borders" (Vol. 1087, pp. 179-185). (Acta Horticulturae; No. 1087).

Dierck, Robrecht ; De Keyser, Ellen ; De Riek, Jan et al. / Hormonal and Genetic Regulation of Axillary Bud Outgrowth in Chrysanthemum morifolium during Floral Initiation. Proceedings of the XXVth International Eucarpia Symposium Section Ornamentals "Crossing Borders". editor / Johan Van Huylenbroeck ; Emmy Dhooghe. Vol. 1087 2015. pp. 179-185 (Acta Horticulturae; 1087).

@inbook{756adbbbbf8d49da9906009dbea10eb5,

title = "Hormonal and Genetic Regulation of Axillary Bud Outgrowth in Chrysanthemum morifolium during Floral Initiation",

abstract = "Shoot branching or the outgrowth of axillary buds is a crucial aspect in the production and breeding of Chrysanthemum sp. The outgrowth of axillary buds is inhibited by the shoot apex through apical dominance. Central to this regulation is the interaction of the plant hormones auxin, cytokinin and strigolactone. In this research we aim to study the hormonal and genetic regulation of apical dominance and axillary bud outgrowth in a cut flower cultivar of Chrysanthemum. For this we combined measurements of axillary bud outgrowth with UPLC-MS/MS quantification of auxin and cytokinin levels and RT-qPCR analysis of two genes involved in the regulation of branching. The cultivar used displayed apical dominance during vegetative growth. A release from apical dominance occurs during floral initiation with outgrowth and elongation of shoots under the apex. The outgrowth of axillary buds after release from apical dominance was preceded by decreased auxin levels in the shoot apex, axillary buds and stem and increased cytokinin levels in the axillary buds. RT-qPCR showed a decreased expression of the local bud outgrowth regulator BRC1 preceding the release from apical dominance at the time of floral initiation.",

author = "Robrecht Dierck and {De Keyser}, Ellen and {De Riek}, Jan and Emmy Dhooghe and {Van Huylenbroeck}, Johan and Els Prinsen and {Van Der Straeten}, Dominique",

year = "2015",

language = "English",

isbn = "9789462610781",

volume = "1087",

series = "Acta Horticulturae",

publisher = "ISHS",

number = "1087",

pages = "179--185",

editor = "{Van Huylenbroeck}, Johan and Emmy Dhooghe",

booktitle = "Proceedings of the XXVth International Eucarpia Symposium Section Ornamentals {"}Crossing Borders{"}",

note = "25th International Eucarpia Symposium Section Ornamentals: Crossing Borders ; Conference date: 28-06-2015 Through 02-07-2015",

}

Dierck, R, De Keyser, E , De Riek, J, Dhooghe, E, Van Huylenbroeck, J, Prinsen, E & Van Der Straeten , D 2015, Hormonal and Genetic Regulation of Axillary Bud Outgrowth in Chrysanthemum morifolium during Floral Initiation. in J Van Huylenbroeck & E Dhooghe (eds), Proceedings of the XXVth International Eucarpia Symposium Section Ornamentals "Crossing Borders". vol. 1087, Acta Horticulturae, no. 1087, pp. 179-185, 25th International Eucarpia Symposium Section Ornamentals: Crossing Borders, Melle, Belgium, 28/06/15.

Hormonal and Genetic Regulation of Axillary Bud Outgrowth in Chrysanthemum morifolium during Floral Initiation. / Dierck, Robrecht; De Keyser, Ellen ; De Riek, Jan et al.

Proceedings of the XXVth International Eucarpia Symposium Section Ornamentals "Crossing Borders". ed. / Johan Van Huylenbroeck; Emmy Dhooghe. Vol. 1087 2015. p. 179-185 (Acta Horticulturae; No. 1087).

Research output: Chapter in Book/Report/Conference proceeding › C1: Articles in proceedings › peer-review

TY - CHAP

T1 - Hormonal and Genetic Regulation of Axillary Bud Outgrowth in Chrysanthemum morifolium during Floral Initiation

AU - Dierck, Robrecht

AU - De Keyser, Ellen

AU - De Riek, Jan

AU - Dhooghe, Emmy

AU - Van Huylenbroeck, Johan

AU - Prinsen, Els

AU - Van Der Straeten , Dominique

PY - 2015

Y1 - 2015

N2 - Shoot branching or the outgrowth of axillary buds is a crucial aspect in the production and breeding of Chrysanthemum sp. The outgrowth of axillary buds is inhibited by the shoot apex through apical dominance. Central to this regulation is the interaction of the plant hormones auxin, cytokinin and strigolactone. In this research we aim to study the hormonal and genetic regulation of apical dominance and axillary bud outgrowth in a cut flower cultivar of Chrysanthemum. For this we combined measurements of axillary bud outgrowth with UPLC-MS/MS quantification of auxin and cytokinin levels and RT-qPCR analysis of two genes involved in the regulation of branching. The cultivar used displayed apical dominance during vegetative growth. A release from apical dominance occurs during floral initiation with outgrowth and elongation of shoots under the apex. The outgrowth of axillary buds after release from apical dominance was preceded by decreased auxin levels in the shoot apex, axillary buds and stem and increased cytokinin levels in the axillary buds. RT-qPCR showed a decreased expression of the local bud outgrowth regulator BRC1 preceding the release from apical dominance at the time of floral initiation.

AB - Shoot branching or the outgrowth of axillary buds is a crucial aspect in the production and breeding of Chrysanthemum sp. The outgrowth of axillary buds is inhibited by the shoot apex through apical dominance. Central to this regulation is the interaction of the plant hormones auxin, cytokinin and strigolactone. In this research we aim to study the hormonal and genetic regulation of apical dominance and axillary bud outgrowth in a cut flower cultivar of Chrysanthemum. For this we combined measurements of axillary bud outgrowth with UPLC-MS/MS quantification of auxin and cytokinin levels and RT-qPCR analysis of two genes involved in the regulation of branching. The cultivar used displayed apical dominance during vegetative growth. A release from apical dominance occurs during floral initiation with outgrowth and elongation of shoots under the apex. The outgrowth of axillary buds after release from apical dominance was preceded by decreased auxin levels in the shoot apex, axillary buds and stem and increased cytokinin levels in the axillary buds. RT-qPCR showed a decreased expression of the local bud outgrowth regulator BRC1 preceding the release from apical dominance at the time of floral initiation.

M3 - C1: Articles in proceedings

SN - 9789462610781

VL - 1087

T3 - Acta Horticulturae

SP - 179

EP - 185

BT - Proceedings of the XXVth International Eucarpia Symposium Section Ornamentals "Crossing Borders"

A2 - Van Huylenbroeck, Johan

A2 - Dhooghe, Emmy

T2 - 25th International Eucarpia Symposium Section Ornamentals: Crossing Borders

Y2 - 28 June 2015 through 2 July 2015

ER -

Dierck R, De Keyser E , De Riek J, Dhooghe E, Van Huylenbroeck J, Prinsen E et al. Hormonal and Genetic Regulation of Axillary Bud Outgrowth in Chrysanthemum morifolium during Floral Initiation. In Van Huylenbroeck J, Dhooghe E, editors, Proceedings of the XXVth International Eucarpia Symposium Section Ornamentals "Crossing Borders". Vol. 1087. 2015. p. 179-185. (Acta Horticulturae; 1087).

Hormonal and Genetic Regulation of Axillary Bud Outgrowth in Chrysanthemum morifolium during Floral Initiation

Abstract

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Projects

IWT-STURING SIERTEELT: Knowledge-driven regulation of plant physiological processes in ornamentals to improve plant quality

Activities

25th International Eucarpia Symposium Section Ornamentals: Crossing Borders

Cite this