TY - JOUR
T1 - Hormone profiling of chrysanthemum hairy roots after co-cultivation with Rhizobium rhizogenes
AU - De Keyser, Ellen
AU - Tyburski, Jarek
AU - Mucha, Natalia
AU - Kesy, Jacek
AU - Eeckhaut, Tom
AU - Dhooghe, Emmy
PY - 2023
Y1 - 2023
N2 - We have used chrysanthemum as a model crop to determine auxin and cytokinin levels in hairy roots formed after coculture of leaf discs with Rhizobium rhizogenes. All 6 genotypes tested formed hairy roots, upon which the presence of T-DNA genes was tested with a qPCR assay, evaluating the presence of TL- as well as TR-genes. Transformation efficiencies varied between 14 and 100%, and for every cultivar, roots with either only TL-genes and roots with both TL- and TR-genes were found. 2-isopentenyladening (2iP) and indole-3-acetic acid (IAA) levels were compared for all 12 roots that yielded sufficient material for analysis. In two cultivars, multiple TL+TR positive independent root lines could be compared, showing a significant difference for as well auxin as cytokinin levels. However, both for 2iP and IAA, 10-fold differences were detected between roots induced on leaves of a single cultivar. This suggests that phytohormone levels, and probably subsequent hairy root regeneration, depend on individual pRi-gene integration for each separate hairy root.
AB - We have used chrysanthemum as a model crop to determine auxin and cytokinin levels in hairy roots formed after coculture of leaf discs with Rhizobium rhizogenes. All 6 genotypes tested formed hairy roots, upon which the presence of T-DNA genes was tested with a qPCR assay, evaluating the presence of TL- as well as TR-genes. Transformation efficiencies varied between 14 and 100%, and for every cultivar, roots with either only TL-genes and roots with both TL- and TR-genes were found. 2-isopentenyladening (2iP) and indole-3-acetic acid (IAA) levels were compared for all 12 roots that yielded sufficient material for analysis. In two cultivars, multiple TL+TR positive independent root lines could be compared, showing a significant difference for as well auxin as cytokinin levels. However, both for 2iP and IAA, 10-fold differences were detected between roots induced on leaves of a single cultivar. This suggests that phytohormone levels, and probably subsequent hairy root regeneration, depend on individual pRi-gene integration for each separate hairy root.
U2 - 10.17660/ActaHortic.2023.1383.25
DO - 10.17660/ActaHortic.2023.1383.25
M3 - A2: International peer reviewed article (not A1-type)
SN - 0567-7572
VL - 1383
SP - 209
EP - 215
JO - Acta Horticulturae
JF - Acta Horticulturae
ER -