Identification of cyst forming nematodes of the genus Heterodera (Nematoda : Heteroderidae) based on the ribosomal DNA-RFLP.

SA Subbotin; Lieven Waeyenberge; M Moens

doi:10.1163/156854100509042

Identification of cyst forming nematodes of the genus Heterodera (Nematoda : Heteroderidae) based on the ribosomal DNA-RFLP.

SA Subbotin
, Lieven Waeyenberge
, M Moens

Research output: Contribution to journal › A1: Web of Science-article › peer-review

Abstract

Amplified ITS region products of rDNA from 25 valid species and one unidentified species from the genus Heterodera and from Meloidodera alni were digested by 26 restriction enzymes. A combination of seven enzymes clearly separated the agriculturally most important species from each other and from their sibling species. Species specific digestion profiles of ITS regions and a table with approximate sizes of digested fragments for several identification enzymes are given. Heterogeneity of ITS regions was revealed for some Cyst forming nematode species.

Original language	English
Journal	Nematology
Volume	2
Issue number	Part 2
Pages (from-to)	153-164
Number of pages	12
ISSN	1388-5545
DOIs	https://doi.org/10.1163/156854100509042
Publication status	Published - 2000

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10.1163/156854100509042

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title = "Identification of cyst forming nematodes of the genus Heterodera (Nematoda : Heteroderidae) based on the ribosomal DNA-RFLP.",

abstract = "Amplified ITS region products of rDNA from 25 valid species and one unidentified species from the genus Heterodera and from Meloidodera alni were digested by 26 restriction enzymes. A combination of seven enzymes clearly separated the agriculturally most important species from each other and from their sibling species. Species specific digestion profiles of ITS regions and a table with approximate sizes of digested fragments for several identification enzymes are given. Heterogeneity of ITS regions was revealed for some Cyst forming nematode species.",

author = "SA Subbotin and Lieven Waeyenberge and M Moens",

year = "2000",

doi = "10.1163/156854100509042",

language = "English",

volume = "2",

pages = "153--164",

journal = "Nematology",

issn = "1388-5545",

publisher = "BRILL ACADEMIC PUBLISHERS",

number = "Part 2",

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T1 - Identification of cyst forming nematodes of the genus Heterodera (Nematoda : Heteroderidae) based on the ribosomal DNA-RFLP.

AU - Subbotin, SA

AU - Waeyenberge, Lieven

AU - Moens, M

PY - 2000

Y1 - 2000

N2 - Amplified ITS region products of rDNA from 25 valid species and one unidentified species from the genus Heterodera and from Meloidodera alni were digested by 26 restriction enzymes. A combination of seven enzymes clearly separated the agriculturally most important species from each other and from their sibling species. Species specific digestion profiles of ITS regions and a table with approximate sizes of digested fragments for several identification enzymes are given. Heterogeneity of ITS regions was revealed for some Cyst forming nematode species.

AB - Amplified ITS region products of rDNA from 25 valid species and one unidentified species from the genus Heterodera and from Meloidodera alni were digested by 26 restriction enzymes. A combination of seven enzymes clearly separated the agriculturally most important species from each other and from their sibling species. Species specific digestion profiles of ITS regions and a table with approximate sizes of digested fragments for several identification enzymes are given. Heterogeneity of ITS regions was revealed for some Cyst forming nematode species.

U2 - 10.1163/156854100509042

DO - 10.1163/156854100509042

M3 - A1: Web of Science-article

SN - 1388-5545

VL - 2

SP - 153

EP - 164

JO - Nematology

JF - Nematology

IS - Part 2

ER -