In vitro induction of tetraploids in ornamental Ranunculus

E. Dhooghe, S. Denis, T. Eeckhaut, D. Reheul, M.C. Van Labeke

    Research output: Contribution to journalA1: Web of Science-articlepeer-review

    Abstract

    This study investigates the capacity of the antimitotic agents colchicine, oryzalin and trifluralin for inducing polyploidisation of Ranunculus asiaticus 'Alfa' in vitro shoots. Flow cytometry was used to evaluate the optimal concentration of each antimitotic agent for polyploidisation. Trifluralin at a concentration of 2 mu M resulted in the highest percentage of polyploidisation (27.5%), followed by a colchicine treatment of 200 mu M, which induced 23.3% of polyploids. For oryzalin the highest percentage was achieved using a concentration of 1 mu M. Different exposure periods were tested and turned out to be an important factor. The maximal exposure period tested (10 weeks) resulted in a significant increase in polyploidisation by oryzalin and trifluralin. In contrast, for colchicine (100 mu M) exposure times of either 16 or 24 h did not significantly influence polyploidisation. Additionally the effect of the antimitotic agents on the viability was analysed. For colchicine no significant effect on the survival rate was observed, for trifluralin only a concentration of 10 mu M affected viability whereas for oryzalin, concentration as well as exposure period were significant parameters. Flow cytometric data were confirmed by counting chromosomes in root tip cells
    Original languageEnglish
    JournalEuphytica
    Volume168
    Issue number1
    Pages (from-to)33-40
    Number of pages8
    ISSN0014-2336
    DOIs
    Publication statusPublished - 2009

    Keywords

    • ADONEUS
    • AGRICULTURE
    • Antimitotic agent
    • Belgium
    • CAPACITY
    • CELLS
    • CHROMOSOMES
    • COLCHICINE
    • COLCHICINE TREATMENT
    • counting
    • DINITROANILINE HERBICIDE
    • DNA
    • EXPOSURE
    • FLOW
    • Flow cytometry
    • FLOW-CYTOMETRY
    • In vitro
    • IN-VITRO
    • INDUCTION
    • NETHERLANDS
    • ORYZALIN
    • PLANT
    • polyploid
    • Polyploidisation
    • POLYPLOIDIZATION
    • polyploids
    • Ranunculaceae
    • Rosa
    • SCIENCE
    • SURVIVAL
    • TETRAPLOIDS
    • TIME
    • Trifluralin
    • TUBULIN
    • VIABILITY

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