In vitro polyploidy induction in Rhododendron simsii hybrids

Tom Eeckhaut; G Samyn; E Van Bockstaele

In vitro polyploidy induction in Rhododendron simsii hybrids

Tom Eeckhaut, G Samyn, E Van Bockstaele

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Research output: Chapter in Book/Report/Conference proceeding › C1: Articles in proceedings

Abstract

In vitro polyploidy induction offers the possibility of quick multiplication over in vivo methods, and is therefore particularly interesting for slowly growing plants. Three different in vitro protocols for the induction of tetraploid Rhododendron simsii hybrids were evaluated. The most successful technique was a daily application of mitotic poison on the apical meristem of seedlings. However, this technique was quite labour intensive. Direct sowing on media enriched with antimitotic agents requires less work but is less efficient. To induce ploidy chimeras starting from an existing genotype, mitotic inhibitors can be added to multiplication media.

Translated title of the contribution	In vitro polyploidy induction in Rhododendron simsii hybrids
Original language	English
Title of host publication	Proceedings of the Twentieth International Eucarpia Symposium - Section Ornamentals: Strategies for New Ornamentals II
Editors	J. Van Huylenbroeck, E Van Bockstaele, P Debergh
Number of pages	7
Publication date	2002
Pages	43-49
ISBN (Print)	978-90-66058-25-5
Publication status	Published - 2002

Keywords

azalea
colchicine
oryzalin
trifluralin
seedlings

Cite this

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title = "In vitro polyploidy induction in Rhododendron simsii hybrids",

abstract = "In vitro polyploidy induction offers the possibility of quick multiplication over in vivo methods, and is therefore particularly interesting for slowly growing plants. Three different in vitro protocols for the induction of tetraploid Rhododendron simsii hybrids were evaluated. The most successful technique was a daily application of mitotic poison on the apical meristem of seedlings. However, this technique was quite labour intensive. Direct sowing on media enriched with antimitotic agents requires less work but is less efficient. To induce ploidy chimeras starting from an existing genotype, mitotic inhibitors can be added to multiplication media. ",

keywords = "azalea, colchicine, oryzalin, trifluralin, seedlings",

author = "Tom Eeckhaut and G Samyn and {Van Bockstaele}, E",

note = "20th International EUCARPIA Symposium - Section Ornamentals, MELLE, BELGIUM, JUL 03-06, 2001",

year = "2002",

language = "English",

isbn = "978-90-66058-25-5 ",

series = "Acta Horticulturae",

publisher = "ISHS",

number = "572",

pages = "43--49",

editor = "{Van Huylenbroeck}, J. and {Van Bockstaele}, E and P Debergh",

booktitle = "Proceedings of the Twentieth International Eucarpia Symposium - Section Ornamentals: Strategies for New Ornamentals II",

}

In vitro polyploidy induction in Rhododendron simsii hybrids. / Eeckhaut, Tom; Samyn, G; Van Bockstaele, E.
Proceedings of the Twentieth International Eucarpia Symposium - Section Ornamentals: Strategies for New Ornamentals II. ed. / J. Van Huylenbroeck; E Van Bockstaele; P Debergh. 2002. p. 43-49 (Acta Horticulturae; No. 572).

Research output: Chapter in Book/Report/Conference proceeding › C1: Articles in proceedings

TY - CHAP

T1 - In vitro polyploidy induction in Rhododendron simsii hybrids

AU - Eeckhaut, Tom

AU - Samyn, G

AU - Van Bockstaele, E

N1 - 20th International EUCARPIA Symposium - Section Ornamentals, MELLE, BELGIUM, JUL 03-06, 2001

PY - 2002

Y1 - 2002

N2 - In vitro polyploidy induction offers the possibility of quick multiplication over in vivo methods, and is therefore particularly interesting for slowly growing plants. Three different in vitro protocols for the induction of tetraploid Rhododendron simsii hybrids were evaluated. The most successful technique was a daily application of mitotic poison on the apical meristem of seedlings. However, this technique was quite labour intensive. Direct sowing on media enriched with antimitotic agents requires less work but is less efficient. To induce ploidy chimeras starting from an existing genotype, mitotic inhibitors can be added to multiplication media.

AB - In vitro polyploidy induction offers the possibility of quick multiplication over in vivo methods, and is therefore particularly interesting for slowly growing plants. Three different in vitro protocols for the induction of tetraploid Rhododendron simsii hybrids were evaluated. The most successful technique was a daily application of mitotic poison on the apical meristem of seedlings. However, this technique was quite labour intensive. Direct sowing on media enriched with antimitotic agents requires less work but is less efficient. To induce ploidy chimeras starting from an existing genotype, mitotic inhibitors can be added to multiplication media.

KW - azalea

KW - colchicine

KW - oryzalin

KW - trifluralin

KW - seedlings

M3 - C1: Articles in proceedings

SN - 978-90-66058-25-5

T3 - Acta Horticulturae

SP - 43

EP - 49

BT - Proceedings of the Twentieth International Eucarpia Symposium - Section Ornamentals: Strategies for New Ornamentals II

A2 - Van Huylenbroeck, J.

A2 - Van Bockstaele, E

A2 - Debergh, P

ER -

In vitro polyploidy induction in Rhododendron simsii hybrids

Abstract

Keywords

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