In vitro somatic embryogenesis and plant regeneration in Zantedeschia hybrids

B. Duquenne; T. Eeckhaut; S. Werbrouck; J. Van Huylenbroeck

doi:10.1007/s11240-006-9161-8

In vitro somatic embryogenesis and plant regeneration in Zantedeschia hybrids

B. Duquenne, T. Eeckhaut, S. Werbrouck, J. Van Huylenbroeck

Research output: Contribution to journal › A1: Web of Science-article › peer-review

Abstract

A method for regeneration of plants from tuber explants of a Zantedeschia hybrid via somatic embryogenesis was developed. In vitro cultures were initiated starting from both anthers and tubers. Somatic embryogenesis was only achieved from tuber explants. 6-Benzyladenine (BA) at 0.6 or 2 mg l(-1) in combination with 2 mg l(-1) alpha-naphthaleneacetic acid (NAA) yielded the highest number of embryos per explant. The somatic embryos converted into plantlets on Murashige and Skoog basal medium supplemented with vitamins, micro- and macronutrients, 1 mg l(-1) 6-tau-tau-(dimethylallylamino)-purine (2iP), 3% sucrose and 0.7% agar. This is the first report on induction of somatic embryogenesis in Zantedeschia

Original language	English
Journal	Plant Cell Tissue and Organ Culture
Volume	87
Issue number	3
Pages (from-to)	329-331
Number of pages	3
ISSN	0167-6857
DOIs	https://doi.org/10.1007/s11240-006-9161-8
Publication status	Published - 2006

Keywords

ACID
Araceae
Belgium
BIOTECHNOLOGY
Breeding
callus formation
CULTURE
CULTURES
EMBRYO
EMBRYOGENESIS
HYBRID
HYBRIDS
In vitro
in vitro culture
IN-VITRO
INDUCTION
MICROBIOLOGY
NETHERLANDS
PLANT
plant regeneration
PLANT-REGENERATION
PLANTS
Regeneration
SCIENCE
somatic embryo induction
somatic embryogenesis
Somatic embryos
tubers

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10.1007/s11240-006-9161-8

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title = "In vitro somatic embryogenesis and plant regeneration in Zantedeschia hybrids",

abstract = "A method for regeneration of plants from tuber explants of a Zantedeschia hybrid via somatic embryogenesis was developed. In vitro cultures were initiated starting from both anthers and tubers. Somatic embryogenesis was only achieved from tuber explants. 6-Benzyladenine (BA) at 0.6 or 2 mg l(-1) in combination with 2 mg l(-1) alpha-naphthaleneacetic acid (NAA) yielded the highest number of embryos per explant. The somatic embryos converted into plantlets on Murashige and Skoog basal medium supplemented with vitamins, micro- and macronutrients, 1 mg l(-1) 6-tau-tau-(dimethylallylamino)-purine (2iP), 3% sucrose and 0.7% agar. This is the first report on induction of somatic embryogenesis in Zantedeschia",

keywords = "ACID, Araceae, Belgium, BIOTECHNOLOGY, Breeding, callus formation, CULTURE, CULTURES, EMBRYO, EMBRYOGENESIS, HYBRID, HYBRIDS, In vitro, in vitro culture, IN-VITRO, INDUCTION, MICROBIOLOGY, NETHERLANDS, PLANT, plant regeneration, PLANT-REGENERATION, PLANTS, Regeneration, SCIENCE, somatic embryo induction, somatic embryogenesis, Somatic embryos, tubers",

author = "B. Duquenne and T. Eeckhaut and S. Werbrouck and {Van Huylenbroeck}, J.",

note = "J English Article Duquenne, B (reprint author), Inst Agr & Fisheries Res, Plant Unit Appl Genet & Breeding, Caritasstr 21, B-9090 Melle, Belgium Barbara.Duquenne@ilvo.vlaanderen.be 8 0 SPRINGER DORDRECHT VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS PLANT CELL TISS ORG DEC Discipline: Biotechnology & Applied Microbiology; Plant Sciences 109DI",

year = "2006",

doi = "10.1007/s11240-006-9161-8",

language = "English",

volume = "87",

pages = "329--331",

journal = "Plant Cell Tissue and Organ Culture",

issn = "0167-6857",

publisher = "Springer Netherlands",

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TY - JOUR

T1 - In vitro somatic embryogenesis and plant regeneration in Zantedeschia hybrids

AU - Duquenne, B.

AU - Eeckhaut, T.

AU - Werbrouck, S.

AU - Van Huylenbroeck, J.

N1 - J English Article Duquenne, B (reprint author), Inst Agr & Fisheries Res, Plant Unit Appl Genet & Breeding, Caritasstr 21, B-9090 Melle, Belgium Barbara.Duquenne@ilvo.vlaanderen.be 8 0 SPRINGER DORDRECHT VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS PLANT CELL TISS ORG DEC Discipline: Biotechnology & Applied Microbiology; Plant Sciences 109DI

PY - 2006

Y1 - 2006

N2 - A method for regeneration of plants from tuber explants of a Zantedeschia hybrid via somatic embryogenesis was developed. In vitro cultures were initiated starting from both anthers and tubers. Somatic embryogenesis was only achieved from tuber explants. 6-Benzyladenine (BA) at 0.6 or 2 mg l(-1) in combination with 2 mg l(-1) alpha-naphthaleneacetic acid (NAA) yielded the highest number of embryos per explant. The somatic embryos converted into plantlets on Murashige and Skoog basal medium supplemented with vitamins, micro- and macronutrients, 1 mg l(-1) 6-tau-tau-(dimethylallylamino)-purine (2iP), 3% sucrose and 0.7% agar. This is the first report on induction of somatic embryogenesis in Zantedeschia

AB - A method for regeneration of plants from tuber explants of a Zantedeschia hybrid via somatic embryogenesis was developed. In vitro cultures were initiated starting from both anthers and tubers. Somatic embryogenesis was only achieved from tuber explants. 6-Benzyladenine (BA) at 0.6 or 2 mg l(-1) in combination with 2 mg l(-1) alpha-naphthaleneacetic acid (NAA) yielded the highest number of embryos per explant. The somatic embryos converted into plantlets on Murashige and Skoog basal medium supplemented with vitamins, micro- and macronutrients, 1 mg l(-1) 6-tau-tau-(dimethylallylamino)-purine (2iP), 3% sucrose and 0.7% agar. This is the first report on induction of somatic embryogenesis in Zantedeschia

KW - ACID

KW - Araceae

KW - Belgium

KW - BIOTECHNOLOGY

KW - Breeding

KW - callus formation

KW - CULTURE

KW - CULTURES

KW - EMBRYO

KW - EMBRYOGENESIS

KW - HYBRID

KW - HYBRIDS

KW - In vitro

KW - in vitro culture

KW - IN-VITRO

KW - INDUCTION

KW - MICROBIOLOGY

KW - NETHERLANDS

KW - PLANT

KW - plant regeneration

KW - PLANT-REGENERATION

KW - PLANTS

KW - Regeneration

KW - SCIENCE

KW - somatic embryo induction

KW - somatic embryogenesis

KW - Somatic embryos

KW - tubers

U2 - 10.1007/s11240-006-9161-8

DO - 10.1007/s11240-006-9161-8

M3 - A1: Web of Science-article

VL - 87

SP - 329

EP - 331

JO - Plant Cell Tissue and Organ Culture

JF - Plant Cell Tissue and Organ Culture

SN - 0167-6857

IS - 3

ER -

In vitro somatic embryogenesis and plant regeneration in Zantedeschia hybrids

Abstract

Keywords

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