In vitro somatic embryogenesis and plant regeneration in Zantedeschia hybrids

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    Abstract

    A method for regeneration of plants from tuber explants of a Zantedeschia hybrid via somatic embryogenesis was developed. In vitro cultures were initiated starting from both anthers and tubers. Somatic embryogenesis was only achieved from tuber explants. 6-Benzyladenine (BA) at 0.6 or 2 mg l(-1) in combination with 2 mg l(-1) alpha-naphthaleneacetic acid (NAA) yielded the highest number of embryos per explant. The somatic embryos converted into plantlets on Murashige and Skoog basal medium supplemented with vitamins, micro- and macronutrients, 1 mg l(-1) 6-tau-tau-(dimethylallylamino)-purine (2iP), 3% sucrose and 0.7% agar. This is the first report on induction of somatic embryogenesis in Zantedeschia
    Original languageEnglish
    JournalPlant Cell Tissue and Organ Culture
    Volume87
    Issue number3
    Pages (from-to)329-331
    Number of pages3
    ISSN0167-6857
    DOIs
    Publication statusPublished - 2006

    Keywords

    • ACID
    • Araceae
    • Belgium
    • BIOTECHNOLOGY
    • Breeding
    • callus formation
    • CULTURE
    • CULTURES
    • EMBRYO
    • EMBRYOGENESIS
    • HYBRID
    • HYBRIDS
    • In vitro
    • in vitro culture
    • IN-VITRO
    • INDUCTION
    • MICROBIOLOGY
    • NETHERLANDS
    • PLANT
    • plant regeneration
    • PLANT-REGENERATION
    • PLANTS
    • Regeneration
    • SCIENCE
    • somatic embryo induction
    • somatic embryogenesis
    • Somatic embryos
    • tubers

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