Induction and conversion of somatic embryogenesis on the anther filament of Spathiphyllum Schott

SPO Werbrouck; Tom Eeckhaut; PC Debergh

Induction and conversion of somatic embryogenesis on the anther filament of Spathiphyllum Schott

SPO Werbrouck
, Tom Eeckhaut
, PC Debergh

Research output: Chapter in Book/Report/Conference proceeding › C1: Articles in proceedings › peer-review

Abstract

A prerequisite for genetic manipulation, mutation breeding or ploidy manipulation of the ornamental crop Spathiphyllum is the availability of a system which allows a target cell to regenerate into a new plant. Induction of somatic embryos could offer a powerful tool to reach this goal. When placed on a Murashige sr Skoog medium supplemented with 10 mu M naphthaleneacetic acid (NAA) and 0.2 - 3 mu M thidiazuron (TDZ), anthers of different genotypes develop clumps of attached somatic embryos on the filament. The younger the anthers, the more sensitive their filament cells were for the induction of somatic embryogenesis. After six weeks, the somatic embryos were transferred to a Murashige Skoog (MS) medium supplemented with 10 CIM benzylaminopurine (BA) + 0.02 mu M NAA to develop secondary somatic embryos. Another 6 weeks later, they were ready for conversion. The best conversion medium was a hormone-free MS medium. Addition of abscisic acid (ABA) had a negative effect. After a conversion period of 10 weeks, the normal looking plants were transferred to the greenhouse.

Translated title of the contribution	Induction and conversion of somatic embryogenesis on the anther filament of Spathiphyllum Schott
Original language	English
Title of host publication	Proceedings of the XXV International Horticultural Congress, Part 10: Application of Biotechnology and Molecular Biology and Breeding In Vitro Culture
Editors	L.H.W. Van der Plas, G.-J. de Klerk
Number of pages	7
Publication date	2000
Pages	263-270
ISBN (Print)	978-90-66058-43-9
Publication status	Published - 2000

Keywords

Spathiphyllum
Araceae
conversion
embryogenesis

Cite this

Werbrouck, SPO., Eeckhaut, T., & Debergh, PC. (2000). Induction and conversion of somatic embryogenesis on the anther filament of Spathiphyllum Schott. In L. H. W. Van der Plas, & G.-J. de Klerk (Eds.), Proceedings of the XXV International Horticultural Congress, Part 10: Application of Biotechnology and Molecular Biology and Breeding In Vitro Culture (pp. 263-270). (Acta Horticulturae; No. 520).

Werbrouck, SPO ; Eeckhaut, Tom ; Debergh, PC. / Induction and conversion of somatic embryogenesis on the anther filament of Spathiphyllum Schott. Proceedings of the XXV International Horticultural Congress, Part 10: Application of Biotechnology and Molecular Biology and Breeding In Vitro Culture. editor / L.H.W. Van der Plas ; G.-J. de Klerk. 2000. pp. 263-270 (Acta Horticulturae; 520).

@inbook{b3ca0a87aa2f4a24a16cbf3ad398cb33,

title = "Induction and conversion of somatic embryogenesis on the anther filament of Spathiphyllum Schott",

abstract = "A prerequisite for genetic manipulation, mutation breeding or ploidy manipulation of the ornamental crop Spathiphyllum is the availability of a system which allows a target cell to regenerate into a new plant. Induction of somatic embryos could offer a powerful tool to reach this goal. When placed on a Murashige sr Skoog medium supplemented with 10 mu M naphthaleneacetic acid (NAA) and 0.2 - 3 mu M thidiazuron (TDZ), anthers of different genotypes develop clumps of attached somatic embryos on the filament. The younger the anthers, the more sensitive their filament cells were for the induction of somatic embryogenesis. After six weeks, the somatic embryos were transferred to a Murashige Skoog (MS) medium supplemented with 10 CIM benzylaminopurine (BA) + 0.02 mu M NAA to develop secondary somatic embryos. Another 6 weeks later, they were ready for conversion. The best conversion medium was a hormone-free MS medium. Addition of abscisic acid (ABA) had a negative effect. After a conversion period of 10 weeks, the normal looking plants were transferred to the greenhouse.",

keywords = "Spathiphyllum, Araceae, conversion, embryogenesis",

author = "SPO Werbrouck and Tom Eeckhaut and PC Debergh",

note = "XXVth International Horticultural Congress, BRUSSELS, BELGIUM, AUG 02-07, 1998",

year = "2000",

language = "English",

isbn = "978-90-66058-43-9",

series = "Acta Horticulturae",

publisher = "ISHS",

number = "520",

pages = "263--270",

editor = "\{Van der Plas\}, L.H.W. and \{de Klerk\}, G.-J.",

booktitle = "Proceedings of the XXV International Horticultural Congress, Part 10: Application of Biotechnology and Molecular Biology and Breeding In Vitro Culture",

}

Werbrouck, SPO, Eeckhaut, T & Debergh, PC 2000, Induction and conversion of somatic embryogenesis on the anther filament of Spathiphyllum Schott. in LHW Van der Plas & G-J de Klerk (eds), Proceedings of the XXV International Horticultural Congress, Part 10: Application of Biotechnology and Molecular Biology and Breeding In Vitro Culture. Acta Horticulturae, no. 520, pp. 263-270.

Induction and conversion of somatic embryogenesis on the anther filament of Spathiphyllum Schott. / Werbrouck, SPO; Eeckhaut, Tom; Debergh, PC.
Proceedings of the XXV International Horticultural Congress, Part 10: Application of Biotechnology and Molecular Biology and Breeding In Vitro Culture. ed. / L.H.W. Van der Plas; G.-J. de Klerk. 2000. p. 263-270 (Acta Horticulturae; No. 520).

Research output: Chapter in Book/Report/Conference proceeding › C1: Articles in proceedings › peer-review

TY - CHAP

T1 - Induction and conversion of somatic embryogenesis on the anther filament of Spathiphyllum Schott

AU - Werbrouck, SPO

AU - Eeckhaut, Tom

AU - Debergh, PC

N1 - XXVth International Horticultural Congress, BRUSSELS, BELGIUM, AUG 02-07, 1998

PY - 2000

Y1 - 2000

N2 - A prerequisite for genetic manipulation, mutation breeding or ploidy manipulation of the ornamental crop Spathiphyllum is the availability of a system which allows a target cell to regenerate into a new plant. Induction of somatic embryos could offer a powerful tool to reach this goal. When placed on a Murashige sr Skoog medium supplemented with 10 mu M naphthaleneacetic acid (NAA) and 0.2 - 3 mu M thidiazuron (TDZ), anthers of different genotypes develop clumps of attached somatic embryos on the filament. The younger the anthers, the more sensitive their filament cells were for the induction of somatic embryogenesis. After six weeks, the somatic embryos were transferred to a Murashige Skoog (MS) medium supplemented with 10 CIM benzylaminopurine (BA) + 0.02 mu M NAA to develop secondary somatic embryos. Another 6 weeks later, they were ready for conversion. The best conversion medium was a hormone-free MS medium. Addition of abscisic acid (ABA) had a negative effect. After a conversion period of 10 weeks, the normal looking plants were transferred to the greenhouse.

AB - A prerequisite for genetic manipulation, mutation breeding or ploidy manipulation of the ornamental crop Spathiphyllum is the availability of a system which allows a target cell to regenerate into a new plant. Induction of somatic embryos could offer a powerful tool to reach this goal. When placed on a Murashige sr Skoog medium supplemented with 10 mu M naphthaleneacetic acid (NAA) and 0.2 - 3 mu M thidiazuron (TDZ), anthers of different genotypes develop clumps of attached somatic embryos on the filament. The younger the anthers, the more sensitive their filament cells were for the induction of somatic embryogenesis. After six weeks, the somatic embryos were transferred to a Murashige Skoog (MS) medium supplemented with 10 CIM benzylaminopurine (BA) + 0.02 mu M NAA to develop secondary somatic embryos. Another 6 weeks later, they were ready for conversion. The best conversion medium was a hormone-free MS medium. Addition of abscisic acid (ABA) had a negative effect. After a conversion period of 10 weeks, the normal looking plants were transferred to the greenhouse.

KW - Spathiphyllum

KW - Araceae

KW - conversion

KW - embryogenesis

M3 - C1: Articles in proceedings

SN - 978-90-66058-43-9

T3 - Acta Horticulturae

SP - 263

EP - 270

BT - Proceedings of the XXV International Horticultural Congress, Part 10: Application of Biotechnology and Molecular Biology and Breeding In Vitro Culture

A2 - Van der Plas, L.H.W.

A2 - de Klerk, G.-J.

ER -

Werbrouck SPO, Eeckhaut T, Debergh PC. Induction and conversion of somatic embryogenesis on the anther filament of Spathiphyllum Schott. In Van der Plas LHW, de Klerk GJ, editors, Proceedings of the XXV International Horticultural Congress, Part 10: Application of Biotechnology and Molecular Biology and Breeding In Vitro Culture. 2000. p. 263-270. (Acta Horticulturae; 520).