Intraspecific genotypic diversity of Bacillus species from raw milk

Valerie De Jonghe; An Coorevits; Joachim Vandroemme; Jeroen Heyrman; Lieve Herman; Paul De Vos; Marc Heyndrickx

doi:10.1016/j.idairyj.2007.11.007

Intraspecific genotypic diversity of Bacillus species from raw milk

Valerie De Jonghe, An Coorevits, Joachim Vandroemme, Jeroen Heyrman, Lieve Herman, Paul De Vos, Marc Heyndrickx

Technology and Food Science

Research output: Contribution to journal › A1: Web of Science-article › peer-review

Abstract

The genotypic diversity of isolates belonging to dominant Bacillus species in raw milk from conventional and organic dairy farms in Belgium was investigated using (GTG)(5) PCR. Three different clusters could be identified in the Bacillus subtilis group, reflecting the intraspecific heterogeneity within this collection of strains. For Bacillus cereus, one predominant type could be detected, indicating a common contamination source or a type that is best capable of contaminating the raw milk. For Bacillus licheniformis, two predominant types were found, that could not be linked to the origin, season or temperature of isolation. The largest diversity was found in the Bacillus pumilus species. Still, one cluster comprising two subclusters could be noticed, that. separated isolates from conventional and organic farms. This study indicates that there is a large difference in the population structure of various important Bacillus species in raw milk. (c) 2007 Elsevier Ltd. All rights reserved.

Original language	English
Journal	International Dairy Journal
Volume	18
Issue number	5
Pages (from-to)	496-505
Number of pages	10
ISSN	0958-6946
DOIs	https://doi.org/10.1016/j.idairyj.2007.11.007
Publication status	Published - 1-May-2008

Access to Document

10.1016/j.idairyj.2007.11.007

Cite this

@article{12825a55851f46e38551f74356a555ce,

title = "Intraspecific genotypic diversity of Bacillus species from raw milk",

abstract = "The genotypic diversity of isolates belonging to dominant Bacillus species in raw milk from conventional and organic dairy farms in Belgium was investigated using (GTG)(5) PCR. Three different clusters could be identified in the Bacillus subtilis group, reflecting the intraspecific heterogeneity within this collection of strains. For Bacillus cereus, one predominant type could be detected, indicating a common contamination source or a type that is best capable of contaminating the raw milk. For Bacillus licheniformis, two predominant types were found, that could not be linked to the origin, season or temperature of isolation. The largest diversity was found in the Bacillus pumilus species. Still, one cluster comprising two subclusters could be noticed, that. separated isolates from conventional and organic farms. This study indicates that there is a large difference in the population structure of various important Bacillus species in raw milk. (c) 2007 Elsevier Ltd. All rights reserved.",

author = "{De Jonghe}, Valerie and An Coorevits and Joachim Vandroemme and Jeroen Heyrman and Lieve Herman and {De Vos}, Paul and Marc Heyndrickx",

year = "2008",

month = may,

day = "1",

doi = "10.1016/j.idairyj.2007.11.007",

language = "English",

volume = "18",

pages = "496--505",

journal = "International Dairy Journal",

issn = "0958-6946",

publisher = "Elsevier BV",

number = "5",

}

TY - JOUR

T1 - Intraspecific genotypic diversity of Bacillus species from raw milk

AU - De Jonghe, Valerie

AU - Coorevits, An

AU - Vandroemme, Joachim

AU - Heyrman, Jeroen

AU - Herman, Lieve

AU - De Vos, Paul

AU - Heyndrickx, Marc

PY - 2008/5/1

Y1 - 2008/5/1

N2 - The genotypic diversity of isolates belonging to dominant Bacillus species in raw milk from conventional and organic dairy farms in Belgium was investigated using (GTG)(5) PCR. Three different clusters could be identified in the Bacillus subtilis group, reflecting the intraspecific heterogeneity within this collection of strains. For Bacillus cereus, one predominant type could be detected, indicating a common contamination source or a type that is best capable of contaminating the raw milk. For Bacillus licheniformis, two predominant types were found, that could not be linked to the origin, season or temperature of isolation. The largest diversity was found in the Bacillus pumilus species. Still, one cluster comprising two subclusters could be noticed, that. separated isolates from conventional and organic farms. This study indicates that there is a large difference in the population structure of various important Bacillus species in raw milk. (c) 2007 Elsevier Ltd. All rights reserved.

AB - The genotypic diversity of isolates belonging to dominant Bacillus species in raw milk from conventional and organic dairy farms in Belgium was investigated using (GTG)(5) PCR. Three different clusters could be identified in the Bacillus subtilis group, reflecting the intraspecific heterogeneity within this collection of strains. For Bacillus cereus, one predominant type could be detected, indicating a common contamination source or a type that is best capable of contaminating the raw milk. For Bacillus licheniformis, two predominant types were found, that could not be linked to the origin, season or temperature of isolation. The largest diversity was found in the Bacillus pumilus species. Still, one cluster comprising two subclusters could be noticed, that. separated isolates from conventional and organic farms. This study indicates that there is a large difference in the population structure of various important Bacillus species in raw milk. (c) 2007 Elsevier Ltd. All rights reserved.

U2 - 10.1016/j.idairyj.2007.11.007

DO - 10.1016/j.idairyj.2007.11.007

M3 - A1: Web of Science-article

SN - 0958-6946

VL - 18

SP - 496

EP - 505

JO - International Dairy Journal

JF - International Dairy Journal

IS - 5

ER -