Isolation and characterization of polymorphic microsatellite markers in Anthyllis vulneraria

Sabine Van Glabeke; Els Coart; Olivier Honnay; Isabel Roldan-Ruiz

doi:10.1111/j.1471-8286.2006.01625.x

Isolation and characterization of polymorphic microsatellite markers in Anthyllis vulneraria

Sabine Van Glabeke, Els Coart, Olivier Honnay, Isabel Roldan-Ruiz

Research output: Contribution to journal › A1: Web of Science-article › peer-review

Abstract

To study the reproductive system of the putative autogamous species Anthyllis vulneraria in Belgian calcareous grasslands, eight polymorphic microsatellite loci were developed using the sequence-tagged microsatellite profiling technique and 35 plants were typed for all loci. No linkage disequilibrium was found between any pair of loci. Over the loci, the number of alleles ranged from two to five. Observed and expected heterozygosities ranged from 0.053 to 0.688 and from 0.053 to 0.631, respectively. The estimated F-IS values (F-IS = 0.031 and 0.122) are not in accordance with a predominantly selfing reproductive system.

Original language	English
Journal	Molecular Ecology Notes
Volume	7
Issue number	3
Pages (from-to)	477-479
Number of pages	3
ISSN	1471-8278
DOIs	https://doi.org/10.1111/j.1471-8286.2006.01625.x
Publication status	Published - 2007

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10.1111/j.1471-8286.2006.01625.x

Cite this

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title = "Isolation and characterization of polymorphic microsatellite markers in Anthyllis vulneraria",

abstract = "To study the reproductive system of the putative autogamous species Anthyllis vulneraria in Belgian calcareous grasslands, eight polymorphic microsatellite loci were developed using the sequence-tagged microsatellite profiling technique and 35 plants were typed for all loci. No linkage disequilibrium was found between any pair of loci. Over the loci, the number of alleles ranged from two to five. Observed and expected heterozygosities ranged from 0.053 to 0.688 and from 0.053 to 0.631, respectively. The estimated F-IS values (F-IS = 0.031 and 0.122) are not in accordance with a predominantly selfing reproductive system.",

author = "{Van Glabeke}, Sabine and Els Coart and Olivier Honnay and Isabel Roldan-Ruiz",

year = "2007",

doi = "10.1111/j.1471-8286.2006.01625.x",

language = "English",

volume = "7",

pages = "477--479",

journal = "Molecular Ecology Resources",

issn = "1755-098X",

publisher = "Wiley-Blackwell",

number = "3",

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T1 - Isolation and characterization of polymorphic microsatellite markers in Anthyllis vulneraria

AU - Van Glabeke, Sabine

AU - Coart, Els

AU - Honnay, Olivier

AU - Roldan-Ruiz, Isabel

PY - 2007

Y1 - 2007

N2 - To study the reproductive system of the putative autogamous species Anthyllis vulneraria in Belgian calcareous grasslands, eight polymorphic microsatellite loci were developed using the sequence-tagged microsatellite profiling technique and 35 plants were typed for all loci. No linkage disequilibrium was found between any pair of loci. Over the loci, the number of alleles ranged from two to five. Observed and expected heterozygosities ranged from 0.053 to 0.688 and from 0.053 to 0.631, respectively. The estimated F-IS values (F-IS = 0.031 and 0.122) are not in accordance with a predominantly selfing reproductive system.

AB - To study the reproductive system of the putative autogamous species Anthyllis vulneraria in Belgian calcareous grasslands, eight polymorphic microsatellite loci were developed using the sequence-tagged microsatellite profiling technique and 35 plants were typed for all loci. No linkage disequilibrium was found between any pair of loci. Over the loci, the number of alleles ranged from two to five. Observed and expected heterozygosities ranged from 0.053 to 0.688 and from 0.053 to 0.631, respectively. The estimated F-IS values (F-IS = 0.031 and 0.122) are not in accordance with a predominantly selfing reproductive system.

U2 - 10.1111/j.1471-8286.2006.01625.x

DO - 10.1111/j.1471-8286.2006.01625.x

M3 - A1: Web of Science-article

VL - 7

SP - 477

EP - 479

JO - Molecular Ecology Resources

JF - Molecular Ecology Resources

SN - 1755-098X

IS - 3

ER -