Ploidy breeding in ornamentals

Tom Eeckhaut, Jeroen Van der Veken, Emmy Dhooghe, Leen Leus, Katrijn Van Laere, Johan Van Huylenbroeck

    Research output: Chapter in Book/Report/Conference proceedingContribution to book/anthologypeer-review


    Ploidy alterations are frequently used in ornamental plant breeding to
    induce novel variation, to overcome crossing barriers, or to create homogenous
    lines. Here we review three in vitro ploidy breeding tools with proven practical
    potential: mitotic polyploidization using mitosis arresting chemicals, meiotic polyploidization through the application of 2n gametes, and haploidization through either male or female gamete regeneration or sexual hybridization techniques. For each tool, we present the state of the art, summarize recent progress, and report on the relative success of different approaches. We summarize the technical procedures of those techniques and their advantages, drawbacks, and repercussions for plant breeding. For mitotic polyploidization, we compare the effects of mitotic inhibitors through meristem treatment, short exposure, and chronic exposure. For unreduced gamete formation via chemicals, we describe N2O exposure and temperature treatment.
    For haploid induction, we compare possibilities of parthenogenesis, microspore
    embryogenesis, and gynogenesis/androgenesis. To conclude, we present the
    perspectives for further research in all of these fields. In the future we expect progress in elucidating molecular backgrounds of 2n gamete formation and their interaction with the environment and the implementation of CENH3 modification as an alternative for classical haploidization techniques. Although our goal is not to provide detailed protocols, we have compiled a basic manual for ploidy breeding. This text gives technical insights into the different induction methods and correlates those to consequences for use in practical breeding.
    Original languageEnglish
    Title of host publicationOrnamental Crops : Handbook of Plant Breeding
    EditorsJohan Van Huylenbroeck
    Publication date2018
    ISBN (Print)978-3-319-90697-3
    Publication statusPublished - 2018


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