Primary structure of a hormonally regulated beta-glucanase of Nicotiana plumbaginifolia

M De Loose, T Alliotte, G Gheysen, C Genetello, J Gielen, P Soetaert, M Van Montagu, D Inzé

    Research output: Contribution to journalA1: Web of Science-articlepeer-review

    Abstract

    A cDNA clone for a hormonally regulated beta-glucanase from Nicotiana plumbaginifolia has been isolated by using an oligodeoxynucleotide probe, synthesized to match the previously determined N-terminal amino acid sequence. The cDNA has the complete sequence of the mature protein and contains at least part of a hydrophobic signal peptide. At the amino acid level, the beta-glucanase of N. plumbaginifolia is 73% homologous to a beta(1,3)-glucanase from tobacco and 52% homologous to a beta(1,3;1,4)-glucanase from barley. Southern-blot analysis clearly demonstrated that N. plumbaginifolia contains at least two related genes encoding beta-glucanase. The extent of the complete signal peptide of the cloned beta-glucanase was determined by sequencing part of the corresponding gene. Northern analysis showed that the expression of the beta-glucanase gene is influenced by auxins and cytokinins.
    Original languageEnglish
    JournalGene
    Volume70
    Issue number1
    Pages (from-to)13-23
    Number of pages11
    ISSN0378-1119
    Publication statusPublished - 1988

    Keywords

    • Amino Acid Sequence
    • Cloning, Molecular
    • Cytokinins
    • DNA
    • Gene Expression Regulation
    • Glucan Endo-1,3-beta-D-Glucosidase
    • Glycoside Hydrolases
    • Molecular Sequence Data
    • Oligonucleotide Probes
    • Plant Growth Regulators
    • Plants, Toxic
    • Restriction Mapping
    • Sequence Homology, Nucleic Acid
    • Tobacco

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