Raman spectroscopic study of bacterial endospores

Joke De Gelder, Patsy Scheldeman, Karen Leus, Marc Heyndrickx, Peter Vandenabeele, Luc Moens, Paul De Vos

    Research output: Contribution to journalA1: Web of Science-article

    Abstract

    Endospores and endospore-forming bacteria were studied by Raman spectroscopy. Raman spectra were recorded from Bacillus licheniformis LMG 7634 at different steps during growth and spore formation, and from spore suspensions obtained from diverse Bacillus and Paenibacillus strains cultured in different conditions (growth media, temperature, peroxide treatment). Raman bands of calcium dipicolinate and amino acids such as phenylalanine and tyrosine are more intense in the spectra of sporulating bacteria compared with those of bacteria from earlier phases of growth. Raman spectroscopy can thus be used to detect sporulation of cells by a characteristic band at 1,018 cm(-1) from calcium dipicolinate. The increase in amino acids could possibly be explained by the formation of small acid-soluble proteins that saturate the endospore DNA. Large variations in Raman spectra of endospore suspensions of different strains or different culturing conditions were observed. Next to calcium dipicolinate, tyrosine and phenylalanine, band differences at 527 and 638 cm(-1) were observed in the spectra of some of the B. sporothermodurans spore suspensions. These bands were assigned to the incorporation of cysteine residues in spore coat proteins. In conclusion, Raman spectroscopy is a fast technique to provide useful information about several spore components.
    Original languageEnglish
    JournalAnalytical and Bioanalytical Chemistry
    Volume389
    Issue number7-8
    Pages (from-to)2143-51
    Number of pages9
    ISSN1618-2642
    DOIs
    Publication statusPublished - 2007

    Keywords

    • Bacillus
    • Bacteriological Techniques
    • Culture Media
    • Spectrum Analysis, Raman
    • Spores, Bacterial

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