Ranking of in vitro fermentability of 20 feedstuffs with an automated gas production technique: Results of a ring test

AH van Gelder, M Hetta, MAM Rodrigues, Johan De Boever, H Den Hartigh, C Rymer, M van Oostrum, R van Kaathoven, JW Cone

    Research output: Contribution to journalA1: Web of Science-articlepeer-review

    Abstract

    A ring test was completed to determine within laboratory repeatability, and reproducibility among laboratories, of a gas production technique for measuring fermentation kinetics of feeds in rumen fluid. Eight laboratories in different European countries received 20 feed samples. All used the same gas production equipment and method. Gas production profiles were fitted to a multiphasic model and the most important parameters were statistically compared. Although the within laboratory repeatability of, and levels of, calculated parameters differed among laboratories, in most cases there was a high correlation between pairs of laboratories (r = 0.93-1.00), although in some cases the correlation was only moderate (r= 0.73-0.87). Within laboratory gas production profiles were influenced by variation in microbiological activity of the rumen fluid and air pressure changes at the end of the run. Among laboratory differences were observed in calibration factors, type of donor animal and diets fed. Variation sources can be minimized using within run standards. (c) 2005 Elsevier B.V. All rights reserved.
    Translated title of the contributionRanking of in vitro fermentability of 20 feedstuffs with an automated gas production technique: Results of a ring test
    Original languageEnglish
    JournalAnimal Feed Science and Technology
    Volume123
    Pages (from-to)243-253
    Number of pages11
    ISSN0377-8401
    DOIs
    Publication statusPublished - 30-Sep-2005

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