Real Time Polymerase Chain Reaction (PCR) quantification of T25 maize seeds: influence of the genetic structures in the maize kernel on the quantitative analysis

Nina Papazova; Isabel Taverniers; Isabelle Degrieck; Erik Van Bockstaele; H.  Joost; Marc De Loose

Real Time Polymerase Chain Reaction (PCR) quantification of T25 maize seeds: influence of the genetic structures in the maize kernel on the quantitative analysis

Nina Papazova, Isabel Taverniers, Isabelle Degrieck, Erik Van Bockstaele, H. Joost, Marc De Loose

Research output: Contribution to journal › A1: Web of Science-article › peer-review

Abstract

Real Time PCR is a technique widely used for GMO quantification. The maize seed contains diploid embryo
and triploid endosperm. This implies that the ratio transgene/endogene determined by Real Time PCR will
vary due to the presence of different genome copies in the seed compartments. The transgene/endogene ratio
is determined for single seeds from commercial transgenic line T25. This ratio is compared between the seeds
that originate from reciprocal crosses between T25 and the conventional variety B73. The variation in the GMO
content due to the presence of seeds with different origin in the seed samples of 3000 seeds is evaluated.

Original language	Dutch
Journal	Seed Science and Technology
Volume	34
Pages (from-to)	307-317
Number of pages	11
Publication status	Published - 2006

Cite this

@article{3b477d8859574a628d01361ab6b7fbe5,

title = "Real Time Polymerase Chain Reaction (PCR) quantification of T25 maize seeds: influence of the genetic structures in the maize kernel on the quantitative analysis",

abstract = "Real Time PCR is a technique widely used for GMO quantification. The maize seed contains diploid embryoand triploid endosperm. This implies that the ratio transgene/endogene determined by Real Time PCR willvary due to the presence of different genome copies in the seed compartments. The transgene/endogene ratiois determined for single seeds from commercial transgenic line T25. This ratio is compared between the seedsthat originate from reciprocal crosses between T25 and the conventional variety B73. The variation in the GMOcontent due to the presence of seeds with different origin in the seed samples of 3000 seeds is evaluated.",

author = "Nina Papazova and Isabel Taverniers and Isabelle Degrieck and {Van Bockstaele}, Erik and H. Joost and {De Loose}, Marc",

year = "2006",

language = "Nederlands",

volume = "34",

pages = "307--317",

journal = "Seed Science and Technology",

issn = "0251-0952",

publisher = "International Seed Testing Association",

}

Real Time Polymerase Chain Reaction (PCR) quantification of T25 maize seeds : influence of the genetic structures in the maize kernel on the quantitative analysis. / Papazova, Nina; Taverniers, Isabel; Degrieck, Isabelle; Van Bockstaele, Erik; Joost, H. ; De Loose, Marc.

In: Seed Science and Technology, Vol. 34, 2006, p. 307-317.

Research output: Contribution to journal › A1: Web of Science-article › peer-review

TY - JOUR

T1 - Real Time Polymerase Chain Reaction (PCR) quantification of T25 maize seeds

T2 - influence of the genetic structures in the maize kernel on the quantitative analysis

AU - Papazova, Nina

AU - Taverniers, Isabel

AU - Degrieck, Isabelle

AU - Van Bockstaele, Erik

AU - Joost, H.

AU - De Loose, Marc

PY - 2006

Y1 - 2006

N2 - Real Time PCR is a technique widely used for GMO quantification. The maize seed contains diploid embryoand triploid endosperm. This implies that the ratio transgene/endogene determined by Real Time PCR willvary due to the presence of different genome copies in the seed compartments. The transgene/endogene ratiois determined for single seeds from commercial transgenic line T25. This ratio is compared between the seedsthat originate from reciprocal crosses between T25 and the conventional variety B73. The variation in the GMOcontent due to the presence of seeds with different origin in the seed samples of 3000 seeds is evaluated.

AB - Real Time PCR is a technique widely used for GMO quantification. The maize seed contains diploid embryoand triploid endosperm. This implies that the ratio transgene/endogene determined by Real Time PCR willvary due to the presence of different genome copies in the seed compartments. The transgene/endogene ratiois determined for single seeds from commercial transgenic line T25. This ratio is compared between the seedsthat originate from reciprocal crosses between T25 and the conventional variety B73. The variation in the GMOcontent due to the presence of seeds with different origin in the seed samples of 3000 seeds is evaluated.

M3 - A1: Web of Science-artikel

VL - 34

SP - 307

EP - 317

JO - Seed Science and Technology

JF - Seed Science and Technology

SN - 0251-0952

ER -