Resistance of red clover to broad spectrum of Sclerotinia trifoliorum

Tim Vleugels; Joost Baert; Kurt Heungens; Marianne Malengier; Gerda Cnops; Erik Van Bockstaele

Resistance of red clover to broad spectrum of Sclerotinia trifoliorum

Tim Vleugels, Joost Baert, Kurt Heungens, Marianne Malengier, Gerda Cnops, Erik Van Bockstaele

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Research output: Chapter in Book/Report/Conference proceeding › C1: Articles in proceedings › peer-review

Abstract

In this research a diversity study on different European isolates of the pathogenic fungus S. trifoliorum will be performed using mycelial compatibility grouping and AFLP. The next step is the development of a bio-test to screen red clover plants for their resistance level against clover rot. The third step in this research is evaluating different European strains for their virulence and evaluating a broad spectrum of red clover varieties for their resistance against S. trifoliorum. Over 100 varieties will be evaluated, including both cultivars, landraces and wild varieties. Finally the inheritage of clover rot resistance in red clover will be evaluated by a QTL study.
Sclerotinia isolates have been collected from clover fields among different European countries. Mycelial compatibility has shown a large variability within fields. The DNA extraction has been optimalized. Sequencing of the ITS-region will be used to determine the exact species of every isolate. Primer combinations are currently being tested for the AFLP study. Different culture media were tested for their capacity to induce the production of multiple big sclerotia. The most optimal medium is being used to produce sclerotia from every isolate. Sclerotia are induced to apothecia formation and formed ascospores will be used to construct the bio-test.

Original language	English
Title of host publication	Sustainable Use of Genetic Diversity in Forage and Turf Breeding, Eucarpia
Number of pages	5
Place of Publication	La Rochelle
Publisher	Humana Press, Springer
Publication date	2009
Pages	375-380
Publication status	Published - 2009
Event	Eucarpia 28th Fodder Crops and Amenity Grasses Section Meeting - La Rochelle, France Duration: 11-May-2009 → 14-May-2009

SCLEROTINIAKLAVER: Improving Sclerotinia resistance to assist red clover breeding
Vleugels, T. (PhD Student) & Baert, J. (Former Project Manager)
1/11/08 → 31/03/13
Project: Research

Cite this

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title = "Resistance of red clover to broad spectrum of Sclerotinia trifoliorum",

abstract = "In this research a diversity study on different European isolates of the pathogenic fungus S. trifoliorum will be performed using mycelial compatibility grouping and AFLP. The next step is the development of a bio-test to screen red clover plants for their resistance level against clover rot. The third step in this research is evaluating different European strains for their virulence and evaluating a broad spectrum of red clover varieties for their resistance against S. trifoliorum. Over 100 varieties will be evaluated, including both cultivars, landraces and wild varieties. Finally the inheritage of clover rot resistance in red clover will be evaluated by a QTL study. Sclerotinia isolates have been collected from clover fields among different European countries. Mycelial compatibility has shown a large variability within fields. The DNA extraction has been optimalized. Sequencing of the ITS-region will be used to determine the exact species of every isolate. Primer combinations are currently being tested for the AFLP study. Different culture media were tested for their capacity to induce the production of multiple big sclerotia. The most optimal medium is being used to produce sclerotia from every isolate. Sclerotia are induced to apothecia formation and formed ascospores will be used to construct the bio-test.",

author = "Tim Vleugels and Joost Baert and Kurt Heungens and Marianne Malengier and Gerda Cnops and {Van Bockstaele}, Erik",

year = "2009",

language = "English",

pages = "375--380",

booktitle = "Sustainable Use of Genetic Diversity in Forage and Turf Breeding, Eucarpia",

publisher = "Humana Press, Springer",

address = "United States",

note = "Eucarpia 28th Fodder Crops and Amenity Grasses Section Meeting ; Conference date: 11-05-2009 Through 14-05-2009",

}

Vleugels, T, Baert, J, Heungens, K , Malengier, M , Cnops, G & Van Bockstaele, E 2009, Resistance of red clover to broad spectrum of Sclerotinia trifoliorum. in Sustainable Use of Genetic Diversity in Forage and Turf Breeding, Eucarpia. Humana Press, Springer, La Rochelle, pp. 375-380, Eucarpia 28th Fodder Crops and Amenity Grasses Section Meeting, La Rochelle, France, 11/05/09.

TY - CHAP

T1 - Resistance of red clover to broad spectrum of Sclerotinia trifoliorum

AU - Vleugels, Tim

AU - Baert, Joost

AU - Heungens, Kurt

AU - Malengier, Marianne

AU - Cnops, Gerda

AU - Van Bockstaele, Erik

PY - 2009

Y1 - 2009

N2 - In this research a diversity study on different European isolates of the pathogenic fungus S. trifoliorum will be performed using mycelial compatibility grouping and AFLP. The next step is the development of a bio-test to screen red clover plants for their resistance level against clover rot. The third step in this research is evaluating different European strains for their virulence and evaluating a broad spectrum of red clover varieties for their resistance against S. trifoliorum. Over 100 varieties will be evaluated, including both cultivars, landraces and wild varieties. Finally the inheritage of clover rot resistance in red clover will be evaluated by a QTL study. Sclerotinia isolates have been collected from clover fields among different European countries. Mycelial compatibility has shown a large variability within fields. The DNA extraction has been optimalized. Sequencing of the ITS-region will be used to determine the exact species of every isolate. Primer combinations are currently being tested for the AFLP study. Different culture media were tested for their capacity to induce the production of multiple big sclerotia. The most optimal medium is being used to produce sclerotia from every isolate. Sclerotia are induced to apothecia formation and formed ascospores will be used to construct the bio-test.

AB - In this research a diversity study on different European isolates of the pathogenic fungus S. trifoliorum will be performed using mycelial compatibility grouping and AFLP. The next step is the development of a bio-test to screen red clover plants for their resistance level against clover rot. The third step in this research is evaluating different European strains for their virulence and evaluating a broad spectrum of red clover varieties for their resistance against S. trifoliorum. Over 100 varieties will be evaluated, including both cultivars, landraces and wild varieties. Finally the inheritage of clover rot resistance in red clover will be evaluated by a QTL study. Sclerotinia isolates have been collected from clover fields among different European countries. Mycelial compatibility has shown a large variability within fields. The DNA extraction has been optimalized. Sequencing of the ITS-region will be used to determine the exact species of every isolate. Primer combinations are currently being tested for the AFLP study. Different culture media were tested for their capacity to induce the production of multiple big sclerotia. The most optimal medium is being used to produce sclerotia from every isolate. Sclerotia are induced to apothecia formation and formed ascospores will be used to construct the bio-test.

M3 - C1: Articles in proceedings

SP - 375

EP - 380

BT - Sustainable Use of Genetic Diversity in Forage and Turf Breeding, Eucarpia

PB - Humana Press, Springer

CY - La Rochelle

T2 - Eucarpia 28th Fodder Crops and Amenity Grasses Section Meeting

Y2 - 11 May 2009 through 14 May 2009

ER -

Resistance of red clover to broad spectrum of Sclerotinia trifoliorum

Abstract

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Projects

SCLEROTINIAKLAVER: Improving Sclerotinia resistance to assist red clover breeding

Cite this