Guidelines for validation of qualitative real-time PCR methods

Sylvia Broeders, Ingrid Huber, Lutz Grohmann, Gilbert Berben, Isabel Taverniers, Marco Mazzara, Nancy Roosens, Dany Morisset

    Research output: Contribution to journalA1: Web of Science-articlepeer-review

    Abstract

    As for many areas of molecular testing, detection of Genetically Modified Organisms (GMO) relies on the real-time Polymerase Chain Reaction (qPCR) technology. Due to the increasing number of GMO, a screening approach using qualitative screening methods has become an integrated part of GMO detection. However, specific guidelines for the validation of these methods are lacking. Here, a pragmatic approach to conduct in-house and inter-laboratory validation studies for GMO screening methods, is proposed. Such guidelines could be adapted to other areas where qualitative qPCR methods are used for molecular testing allowing to implement easily a more reliable screening phase where necessary.
    Translated title of the contributionRichtlijnen voor validatie van kwalitatieve real-time PCR methoden
    Original languageEnglish
    JournalTrends in Food Science and Technology
    Volume37
    Issue number2
    Pages (from-to)115-126
    Number of pages12
    ISSN0924-2244
    Publication statusPublished - 30-Jun-2014

    Keywords

    • B420-nutrition

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