Stability of the T-DNA flanking regions in transgenic Arabidopsis thaliana plants under influence of abiotic stress and cultivation practices

Nina Papazova, Rim Ghedira, Sabine Van Glabeke, Aghleb Bartegi, Pieter Windels, Isabel Taverniers, Isabel Roldan-Ruiz, Erik Van Bockstaele, Anne Milcamps, Guy Van den Eede, Ann Depicker, Marc De Loose

Research output: Contribution to journalA1: Web of Science-articlepeer-review

Abstract

Genetic transformation is often associated with different rearrangements of the plant genome at the site of insertion. Therefore the question remains weather these T-DNA insertion sites are more prone to genotoxic stresses. Here, we studied the impact of propagation through generations, the influence of gene stacking and of photo oxidative stress caused by high light intensity on the stability of the transgene flanking regions in the model plant Arabidopsis thaliana. Conformational Sensitive Capillary Electrophoresis (CSCE), RFLP and sequencing were deployed in this analysis in order to study the proximal 100 bp and the long-range T-DNA flanking sequences. By screening seven transgenic lines no evidence for occurrence of mutation events were found, implying that the nucleotide sequence of the T-DNA flanking regions of the studied events is unlikely to be unstable.
Original languageEnglish
JournalPlant Cell Reports
Volume27
Issue number4
Pages (from-to)749-757
Number of pages9
ISSN0721-7714
DOIs
Publication statusPublished - 2008

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