Sugar beet seed architecture and genetics: Influence on DNA-based GM quantification

Nina Papazova, Isabel Taverniers, Erik Van Bockstaele, Marc De Loose

Research output: Contribution to journalA1: Web of Science-articlepeer-review

Abstract

GMO contaminations in seeds are quantified either as a proportion if transgenic seeds to the total number of seeds or as a proportion of transgenic genomes of the total genome number. We quantified the DNA copy numbers in the embryo and pericarp of sugar beet seeds from three diploid and three triploid varieties. The embryo appears to contain more DNA than the pericarp although it has a lower mass proportion in the seed. The embryo is formed after a fusion of the female and male gametes and contains genome sets from both parents whilst the pericarp stems from the female parent. We estimated the influence of the origin of the transgene and the ploidy of the embryo on the transgenic genome proportion in hemizygous sugar beet seeds and compared transgenic genome numbers with transgenic seed numbers. The results show a significant influence of the biological factors on the transgenic genome proportions which further contributes to the impossibility to convert transgenic genomes in transgenic seeds and vice versa.
Original languageEnglish
JournalSeed Science and Technology
Volume36
Pages (from-to)546-554
Number of pages9
Publication statusPublished - 2008

Keywords

  • B390-plant-breeding
  • B390-breeding
  • P320-nucleic-acids

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