Test performance study of isothermal ampliﬁcation tests for the detection of grapevine ﬂavescence dorée phytoplasma and ‘Candidatus Phytoplasma solani’

Natasa Mehle; Polona  Kogovsek; Fiona Constable; Kris De Jonghe; Marianne Loiseau; Flavio Veratti; Cristina Marzachi; Lucca Ferretti; Elisa Angelini; Luisa Filippin; Esmeraldina Sousa; Eugenia Maria de Andrade; Jennifer Hodgetts; Matt Dickinson; Marina Dermastia

doi:10.1111/epp.12351

Test performance study of isothermal ampliﬁcation tests for the detection of grapevine ﬂavescence dorée phytoplasma and ‘Candidatus Phytoplasma solani’

Natasa Mehle, Polona Kogovsek, Fiona Constable, Kris De Jonghe, Marianne Loiseau, Flavio Veratti, Cristina Marzachi, Lucca Ferretti, Elisa Angelini, Luisa Filippin, Esmeraldina Sousa, Eugenia Maria de Andrade, Jennifer Hodgetts, Matt Dickinson, Marina Dermastia

Research output: Contribution to journal › A2: International peer reviewed article (not A1-type) › peer-review

Abstract

This test performance study (TPS) was carried out on DNA samples from grapevine, clematis, fungi and bacteria to compare and validate loop-mediated isothermal amplification (LAMP) tests for detection of Grapevine flavescence dorée phytoplasma and ‘Candidatus Phytoplasma solani’ (Grapevine Bois noir phytoplasma). Two LAMP tests, for Grapevine flavescence dorée phytoplasma and ‘Candidatus Phytoplasma solani’ (as developed by Kogovšek and colleagues), with proven applicability for rapid laboratory or on-site detection were included in this study. They were performed in 10 laboratories. In addition, the commercial Qualiplante/Hyris isothermal amplification test for Grapevine flavescence dorée phytoplasma was performed in three laboratories. The accuracy of the three tests was shown to be over 98%. Moreover, the high accuracy of these tests, which used different devices across different laboratories, confirmed their reproducibility.

Original language	English
Journal	EPPO Bulletin
Volume	47
Issue number	1
Pages (from-to)	18-23
Number of pages	6
ISSN	0250-8052
DOIs	https://doi.org/10.1111/epp.12351
Publication status	Published - 24-Jan-2017

Keywords

B390-phytopathology

Access to Document

10.1111/epp.12351

1 Finished

WEB_STATUS: Studying the status of quarantine and alert organisms in plants
De Jonghe, K., Heungens, K., Van Vaerenbergh, J., Viaene, N., Maes, M. & Casteels, H.
1/01/09 → 31/12/17
Project: Research

Cite this

Mehle, N., Kogovsek, P., Constable, F., De Jonghe, K., Loiseau, M., Veratti, F., Marzachi, C., Ferretti, L., Angelini, E., Filippin, L., Sousa, E., Andrade, E. M. D., Hodgetts, J., Dickinson, M., & Dermastia, M. (2017). Test performance study of isothermal ampliﬁcation tests for the detection of grapevine ﬂavescence dorée phytoplasma and ‘Candidatus Phytoplasma solani’. EPPO Bulletin, 47(1), 18-23. https://doi.org/10.1111/epp.12351

Mehle, Natasa ; Kogovsek, Polona ; Constable, Fiona ; De Jonghe, Kris ; Loiseau, Marianne ; Veratti, Flavio ; Marzachi, Cristina ; Ferretti, Lucca ; Angelini, Elisa ; Filippin, Luisa ; Sousa, Esmeraldina ; Andrade, Eugenia Maria de ; Hodgetts, Jennifer ; Dickinson, Matt ; Dermastia, Marina. / Test performance study of isothermal ampliﬁcation tests for the detection of grapevine ﬂavescence dorée phytoplasma and ‘Candidatus Phytoplasma solani’. In: EPPO Bulletin. 2017 ; Vol. 47, No. 1. pp. 18-23.

@article{d1bc77c88ab8496aa77990d8404c5c3c,

title = "Test performance study of isothermal ampliﬁcation tests for the detection of grapevine ﬂavescence dor{\'e}e phytoplasma and {\textquoteleft}Candidatus Phytoplasma solani{\textquoteright}",

abstract = "This test performance study (TPS) was carried out on DNA samples from grapevine, clematis, fungi and bacteria to compare and validate loop-mediated isothermal amplification (LAMP) tests for detection of Grapevine flavescence dor{\'e}e phytoplasma and {\textquoteleft}Candidatus Phytoplasma solani{\textquoteright} (Grapevine Bois noir phytoplasma). Two LAMP tests, for Grapevine flavescence dor{\'e}e phytoplasma and {\textquoteleft}Candidatus Phytoplasma solani{\textquoteright} (as developed by Kogov{\v s}ek and colleagues), with proven applicability for rapid laboratory or on-site detection were included in this study. They were performed in 10 laboratories. In addition, the commercial Qualiplante/Hyris isothermal amplification test for Grapevine flavescence dor{\'e}e phytoplasma was performed in three laboratories. The accuracy of the three tests was shown to be over 98%. Moreover, the high accuracy of these tests, which used different devices across different laboratories, confirmed their reproducibility.",

keywords = "B390-phytopathology",

author = "Natasa Mehle and Polona Kogovsek and Fiona Constable and {De Jonghe}, Kris and Marianne Loiseau and Flavio Veratti and Cristina Marzachi and Lucca Ferretti and Elisa Angelini and Luisa Filippin and Esmeraldina Sousa and Andrade, {Eugenia Maria de} and Jennifer Hodgetts and Matt Dickinson and Marina Dermastia",

year = "2017",

month = jan,

day = "24",

doi = "10.1111/epp.12351",

language = "English",

volume = "47",

pages = "18--23",

journal = "EPPO Bulletin",

issn = "0250-8052",

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}

Mehle, N, Kogovsek, P, Constable, F, De Jonghe, K, Loiseau, M, Veratti, F, Marzachi, C, Ferretti, L, Angelini, E, Filippin, L, Sousa, E, Andrade, EMD, Hodgetts, J, Dickinson, M & Dermastia, M 2017, 'Test performance study of isothermal ampliﬁcation tests for the detection of grapevine ﬂavescence dorée phytoplasma and ‘Candidatus Phytoplasma solani’', EPPO Bulletin, vol. 47, no. 1, pp. 18-23. https://doi.org/10.1111/epp.12351

Test performance study of isothermal ampliﬁcation tests for the detection of grapevine ﬂavescence dorée phytoplasma and ‘Candidatus Phytoplasma solani’. / Mehle, Natasa; Kogovsek, Polona ; Constable, Fiona; De Jonghe, Kris; Loiseau, Marianne; Veratti, Flavio; Marzachi, Cristina; Ferretti, Lucca; Angelini, Elisa; Filippin, Luisa; Sousa, Esmeraldina; Andrade, Eugenia Maria de; Hodgetts, Jennifer; Dickinson, Matt; Dermastia, Marina.

In: EPPO Bulletin, Vol. 47, No. 1, 24.01.2017, p. 18-23.

Research output: Contribution to journal › A2: International peer reviewed article (not A1-type) › peer-review

TY - JOUR

T1 - Test performance study of isothermal ampliﬁcation tests for the detection of grapevine ﬂavescence dorée phytoplasma and ‘Candidatus Phytoplasma solani’

AU - Mehle, Natasa

AU - Kogovsek, Polona

AU - Constable, Fiona

AU - De Jonghe, Kris

AU - Loiseau, Marianne

AU - Veratti, Flavio

AU - Marzachi, Cristina

AU - Ferretti, Lucca

AU - Angelini, Elisa

AU - Filippin, Luisa

AU - Sousa, Esmeraldina

AU - Andrade, Eugenia Maria de

AU - Hodgetts, Jennifer

AU - Dickinson, Matt

AU - Dermastia, Marina

PY - 2017/1/24

Y1 - 2017/1/24

N2 - This test performance study (TPS) was carried out on DNA samples from grapevine, clematis, fungi and bacteria to compare and validate loop-mediated isothermal amplification (LAMP) tests for detection of Grapevine flavescence dorée phytoplasma and ‘Candidatus Phytoplasma solani’ (Grapevine Bois noir phytoplasma). Two LAMP tests, for Grapevine flavescence dorée phytoplasma and ‘Candidatus Phytoplasma solani’ (as developed by Kogovšek and colleagues), with proven applicability for rapid laboratory or on-site detection were included in this study. They were performed in 10 laboratories. In addition, the commercial Qualiplante/Hyris isothermal amplification test for Grapevine flavescence dorée phytoplasma was performed in three laboratories. The accuracy of the three tests was shown to be over 98%. Moreover, the high accuracy of these tests, which used different devices across different laboratories, confirmed their reproducibility.

AB - This test performance study (TPS) was carried out on DNA samples from grapevine, clematis, fungi and bacteria to compare and validate loop-mediated isothermal amplification (LAMP) tests for detection of Grapevine flavescence dorée phytoplasma and ‘Candidatus Phytoplasma solani’ (Grapevine Bois noir phytoplasma). Two LAMP tests, for Grapevine flavescence dorée phytoplasma and ‘Candidatus Phytoplasma solani’ (as developed by Kogovšek and colleagues), with proven applicability for rapid laboratory or on-site detection were included in this study. They were performed in 10 laboratories. In addition, the commercial Qualiplante/Hyris isothermal amplification test for Grapevine flavescence dorée phytoplasma was performed in three laboratories. The accuracy of the three tests was shown to be over 98%. Moreover, the high accuracy of these tests, which used different devices across different laboratories, confirmed their reproducibility.

KW - B390-phytopathology

U2 - 10.1111/epp.12351

DO - 10.1111/epp.12351

M3 - A2: International peer reviewed article (not A1-type)

VL - 47

SP - 18

EP - 23

JO - EPPO Bulletin

JF - EPPO Bulletin

SN - 0250-8052

IS - 1

ER -

Test performance study of isothermal ampliﬁcation tests for the detection of grapevine ﬂavescence dorée phytoplasma and ‘Candidatus Phytoplasma solani’

Abstract

Keywords

Access to Document

Projects

WEB_STATUS: Studying the status of quarantine and alert organisms in plants

Cite this