Test performance study of isothermal amplification tests for the detection of grapevine flavescence dorée phytoplasma and ‘Candidatus Phytoplasma solani’

Natasa Mehle, Polona Kogovsek, Fiona Constable, Kris De Jonghe, Marianne Loiseau, Flavio Veratti, Cristina Marzachi, Lucca Ferretti, Elisa Angelini, Luisa Filippin, Esmeraldina Sousa, Eugenia Maria de Andrade, Jennifer Hodgetts, Matt Dickinson, Marina Dermastia

    Research output: Contribution to journalA2: International peer reviewed article (not A1-type)peer-review

    Abstract

    This test performance study (TPS) was carried out on DNA samples from grapevine, clematis, fungi and bacteria to compare and validate loop-mediated isothermal amplification (LAMP) tests for detection of Grapevine flavescence dorée phytoplasma and ‘Candidatus Phytoplasma solani’ (Grapevine Bois noir phytoplasma). Two LAMP tests, for Grapevine flavescence dorée phytoplasma and ‘Candidatus Phytoplasma solani’ (as developed by Kogovšek and colleagues), with proven applicability for rapid laboratory or on-site detection were included in this study. They were performed in 10 laboratories. In addition, the commercial Qualiplante/Hyris isothermal amplification test for Grapevine flavescence dorée phytoplasma was performed in three laboratories. The accuracy of the three tests was shown to be over 98%. Moreover, the high accuracy of these tests, which used different devices across different laboratories, confirmed their reproducibility.
    Original languageEnglish
    JournalEPPO Bulletin
    Volume47
    Issue number1
    Pages (from-to)18-23
    Number of pages6
    ISSN0250-8052
    DOIs
    Publication statusPublished - 24-Jan-2017

    Keywords

    • B390-phytopathology

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