The extensin signal peptide allows secretion of a heterologous protein from protoplasts

M De Loose, G Gheysen, C Tiré, J Gielen, R Villarroel, C Genetello, M Van Montagu, A Depicker, D Inzé

    Research output: Contribution to journalA1: Web of Science-articlepeer-review


    Extensins are hydroxyproline-rich glycoproteins which are amongst the most abundant proteins present in the cell wall of higher plants. Here, we describe the structural analysis of an extensin-encoding gene from Nicotiana plumbaginifolia. The encoded protein (46 kDa) has a highly repetitive structure and contains 37% proline, 18.1% tyrosine, 13.4% lysine, 8.1% serine and 7.1% histidine. The extensin-encoding sequence contains a typical signal peptide for translocation of the protein to the endoplasmic reticulum. By using chimeric genes consisting of different 5' parts of the extensin-encoding gene and the neomycin phosphotransferase II-encoding gene (nptII) as reporter gene, we show that the N-terminal part of extensin can mediate the secretion of NPTII from electroporated N. tabacum protoplasts.
    Original languageEnglish
    Issue number1
    Pages (from-to)95-100
    Number of pages6
    Publication statusPublished - 1991


    • Amino Acid Sequence
    • Base Sequence
    • Genes, Plant
    • Glycoproteins
    • Molecular Sequence Data
    • Plant Proteins
    • Plants, Toxic
    • Protein Conformation
    • Protein Sorting Signals
    • Protoplasts
    • Repetitive Sequences, Nucleic Acid
    • Restriction Mapping
    • Tobacco


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