The impact of MIQE guidelines in Plant Science Community

    Research output: Chapter in Book/Report/Conference proceedingC3: Conference Abstract

    Abstract

    Currently in plant research, validated reliable RT-qPCR protocols are still rare. The methods used are often not at all performed according to the MIQE-guidelines. The necessity of using multiple reference genes has become obvious in quite some cases, but assay-specific validation of these genes is often lacking. Also RNA quality control is a crucial bottleneck. Machines for capillary electrophoresis allow determining RNA quality quite easily, but RIN or RQI values do not apply on plant material. The use of noRT samples is another delicate point. In our experience, the appearance of samples of which the Cq-value of the noRT is within 5 units of the actual sample is common in most experiments. Ignoring this information can lead to a severe overestimation of the gene expression in a specific sample. These three problems will be discussed more profoundly in view of the necessary applicaton of the MIQE-guidelines in plant research.
    Original languageEnglish
    Title of host publicationGlobal Engage's qPCR and Digital PCR Congress
    Number of pages1
    Place of PublicationLyon
    Publication dateSep-2013
    Publication statusPublished - Sep-2013
    EventqPCR and Digital PCR Congress - Lyon, France
    Duration: 9-Sep-201310-Sep-2013

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