Ultrahigh-performance liquid chromatographic-tandem mass spectrometric multimycotoxin method for quantitating 26 mycotoxins in maize silage

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    Abstract

    A multianalyte method was developed to identify and quantitate 26 mycotoxins simultaneously in maize silage by means of ultrahigh-performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS). The extraction and cleanup procedure consists of two extraction steps followed by purification on a Waters Oasis HLB column. The method developed was validated with the requirements of Commission Decision 2002/657/EC taken into account. The limit of detection and quantitation ranges were 5-348 and 11-695 ng/g, respectively. Apparent recovery varied between 61 and 116%, whereas repeatability and reproducibility were within the ranges of 3-45 and 5-49%, respectively. The method developed was successfully applied for maize silage samples taken at the cutting surface and 1 m behind that surface. Mainly Fusarium toxins (beauvericin, deoxynivalenol, enniatins, fumonisins, fusaric acid, and zearalenone) were detected, but postharvest toxins such as mycophenolic acid and roquefortine C were identified as well.
    Original languageEnglish
    JournalJournal of Agricultural and Food Chemistry
    Volume59
    Issue number18
    Pages (from-to)9747-55
    Number of pages9
    ISSN0021-8561
    DOIs
    Publication statusPublished - 2011

    Keywords

    • Chromatography, High Pressure Liquid
    • Food Contamination
    • Mycotoxins
    • Reproducibility of Results
    • Silage
    • Tandem Mass Spectrometry
    • Zea mays

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