Validation of reference genes for gene expression analysis in chicory (Cichorium intybus) using quantitative real-time PCR

Asad Maroufi, Erik Van Bockstaele, Marc De Loose

Research output: Contribution to journalA1: Web of Science-article

Abstract

Quantitative real-time reverse transcriptase polymerase chain reaction (qRT-PCR) is a sensitive technique for quantifying gene expression levels. One or more appropriate reference genes must be selected to accurately compare mRNA transcripts across different samples and tissues. Thus far, only actin-2 has been used as a reference gene for qRT-PCR in chicory, and a full comparison of several candidate reference genes in chicory has not yet been reported.
Original languageEnglish
JournalBMC Molecular Biology
Volume11
Pages (from-to)15
ISSN1471-2199
DOIs
Publication statusPublished - 2010

Keywords

  • Actins
  • Chicory
  • Gene Expression Profiling
  • Genes, Plant
  • Peptide Elongation Factor 1
  • Plant Leaves
  • Plant Roots
  • Polymerase Chain Reaction
  • RNA, Ribosomal, 18S
  • Reference Standards

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