Validation of reference genes for gene expression analysis in chicory (Cichorium intybus) using quantitative real-time PCR

Asad Maroufi, Erik Van Bockstaele, Marc De Loose

Research output: Contribution to journalA1: Web of Science-article


Quantitative real-time reverse transcriptase polymerase chain reaction (qRT-PCR) is a sensitive technique for quantifying gene expression levels. One or more appropriate reference genes must be selected to accurately compare mRNA transcripts across different samples and tissues. Thus far, only actin-2 has been used as a reference gene for qRT-PCR in chicory, and a full comparison of several candidate reference genes in chicory has not yet been reported.
Original languageEnglish
JournalBMC Molecular Biology
Pages (from-to)15
Publication statusPublished - 2010


  • Actins
  • Chicory
  • Gene Expression Profiling
  • Genes, Plant
  • Peptide Elongation Factor 1
  • Plant Leaves
  • Plant Roots
  • Polymerase Chain Reaction
  • RNA, Ribosomal, 18S
  • Reference Standards


Dive into the research topics of 'Validation of reference genes for gene expression analysis in chicory (<em>Cichorium intybus</em>) using quantitative real-time PCR'. Together they form a unique fingerprint.

Cite this