Validation of reference genes for gene expression analysis in chicory (Cichorium intybus) using quantitative real-time PCR

Asad Maroufi; Erik Van Bockstaele; Marc De Loose

doi:10.1186/1471-2199-11-15

Validation of reference genes for gene expression analysis in chicory (Cichorium intybus) using quantitative real-time PCR

Asad Maroufi
, Erik Van Bockstaele
, Marc De Loose

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Research output: Contribution to journal › A1: Web of Science-article › peer-review

Abstract

Quantitative real-time reverse transcriptase polymerase chain reaction (qRT-PCR) is a sensitive technique for quantifying gene expression levels. One or more appropriate reference genes must be selected to accurately compare mRNA transcripts across different samples and tissues. Thus far, only actin-2 has been used as a reference gene for qRT-PCR in chicory, and a full comparison of several candidate reference genes in chicory has not yet been reported.

Original language	English
Journal	BMC Molecular Biology
Volume	11
Pages (from-to)	15
ISSN	1471-2199
DOIs	https://doi.org/10.1186/1471-2199-11-15
Publication status	Published - 2010

Keywords

Actins
Chicory
Gene Expression Profiling
Genes, Plant
Peptide Elongation Factor 1
Plant Leaves
Plant Roots
Polymerase Chain Reaction
RNA, Ribosomal, 18S
Reference Standards

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10.1186/1471-2199-11-15

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title = "Validation of reference genes for gene expression analysis in chicory (Cichorium intybus) using quantitative real-time PCR",

abstract = "Quantitative real-time reverse transcriptase polymerase chain reaction (qRT-PCR) is a sensitive technique for quantifying gene expression levels. One or more appropriate reference genes must be selected to accurately compare mRNA transcripts across different samples and tissues. Thus far, only actin-2 has been used as a reference gene for qRT-PCR in chicory, and a full comparison of several candidate reference genes in chicory has not yet been reported.",

keywords = "Actins, Chicory, Gene Expression Profiling, Genes, Plant, Peptide Elongation Factor 1, Plant Leaves, Plant Roots, Polymerase Chain Reaction, RNA, Ribosomal, 18S, Reference Standards",

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year = "2010",

doi = "10.1186/1471-2199-11-15",

language = "English",

volume = "11",

pages = "15",

journal = "BMC Molecular Biology",

issn = "1471-2199",

publisher = "BioMed Central Ltd.",

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TY - JOUR

T1 - Validation of reference genes for gene expression analysis in chicory (Cichorium intybus) using quantitative real-time PCR

AU - Maroufi, Asad

AU - Van Bockstaele, Erik

AU - De Loose, Marc

PY - 2010

Y1 - 2010

N2 - Quantitative real-time reverse transcriptase polymerase chain reaction (qRT-PCR) is a sensitive technique for quantifying gene expression levels. One or more appropriate reference genes must be selected to accurately compare mRNA transcripts across different samples and tissues. Thus far, only actin-2 has been used as a reference gene for qRT-PCR in chicory, and a full comparison of several candidate reference genes in chicory has not yet been reported.

AB - Quantitative real-time reverse transcriptase polymerase chain reaction (qRT-PCR) is a sensitive technique for quantifying gene expression levels. One or more appropriate reference genes must be selected to accurately compare mRNA transcripts across different samples and tissues. Thus far, only actin-2 has been used as a reference gene for qRT-PCR in chicory, and a full comparison of several candidate reference genes in chicory has not yet been reported.

KW - Actins

KW - Chicory

KW - Gene Expression Profiling

KW - Genes, Plant

KW - Peptide Elongation Factor 1

KW - Plant Leaves

KW - Plant Roots

KW - Polymerase Chain Reaction

KW - RNA, Ribosomal, 18S

KW - Reference Standards

U2 - 10.1186/1471-2199-11-15

DO - 10.1186/1471-2199-11-15

M3 - A1: Web of Science-article

C2 - 20156357

SN - 1471-2199

VL - 11

SP - 15

JO - BMC Molecular Biology

JF - BMC Molecular Biology

ER -

Validation of reference genes for gene expression analysis in chicory (Cichorium intybus) using quantitative real-time PCR

Abstract

Keywords

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