Validation of the microbiological inhibitor test Charm Blue Yellow II for screening for antimicrobials in milk

Wim Reybroeck; Sigrid Ooghe

Validation of the microbiological inhibitor test Charm Blue Yellow II for screening for antimicrobials in milk

Research output: Chapter in Book/Report/Conference proceeding › C1: Articles in proceedings

Abstract

The Charm Blue Yellow II (Charm Sciences Inc., Lawrence, MA) is a broad-spectrum microbiological inhibitor test for the detection of antimicrobials in milk. The test was validated at ILVO-T&V according to Commission Decision 2002/657/EC.
All ß-lactam antibiotics were detected at their respective MRL, except for desfuroylceftiofur. Moreover, all tetracyclines, 3 out of 5 sulfonamides and also tylosin, tilmicosin, gentamycin, neomycin, lincomycin and pirlimycin were detected at their respective MRL.
The repeatability in scores for the Charm Blue Yellow II is not always as good as the repeatability in colour factors obtained for other similar microbiological tests.
Interferences (false positive results) were observed for high somatic cell milk, for milk with a high fat content and for milk with a low or high protein content. These results are consistent with other inhibitory tests evaluated, with the exception of CMT (Copan Milk Test).

Original language	English
Title of host publication	Residues of veterinary drugs in food : Proceedings of the EuroResidue VII Conference, Egmond aan Zee, The Netherlands, 14-16 May 2012
Editors	Robert Schilt
Number of pages	6
Volume	2
Publication date	2012
Pages	563-568
ISBN (Print)	978-94-6173-318-4
Publication status	Published - 2012
Event	EuroResidue VII Conference on Residues of Veterinary Drugs in Food - Egmond aan Zee, Egmond aan Zee, Netherlands Duration: 14-May-2012 → 16-May-2012

1 Finished
1 Active

VALIDATIEANTIBIO: Validation of screening tests of antibiotic residues for kit manufacturers
Reybroeck, W., Heyndrickx, M. & Ooghe, S.
1/01/10 → …
Project: Research
DETTECH: Development and validation of new methods for detection of residues
Reybroeck, W. & Ooghe, S.
1/01/95 → 31/12/17
Project: Research

Cite this

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title = "Validation of the microbiological inhibitor test Charm Blue Yellow II for screening for antimicrobials in milk",

abstract = "The Charm Blue Yellow II (Charm Sciences Inc., Lawrence, MA) is a broad-spectrum microbiological inhibitor test for the detection of antimicrobials in milk. The test was validated at ILVO-T&V according to Commission Decision 2002/657/EC. All {\ss}-lactam antibiotics were detected at their respective MRL, except for desfuroylceftiofur. Moreover, all tetracyclines, 3 out of 5 sulfonamides and also tylosin, tilmicosin, gentamycin, neomycin, lincomycin and pirlimycin were detected at their respective MRL. The repeatability in scores for the Charm Blue Yellow II is not always as good as the repeatability in colour factors obtained for other similar microbiological tests. Interferences (false positive results) were observed for high somatic cell milk, for milk with a high fat content and for milk with a low or high protein content. These results are consistent with other inhibitory tests evaluated, with the exception of CMT (Copan Milk Test). ",

author = "Wim Reybroeck and Sigrid Ooghe",

year = "2012",

language = "English",

isbn = "978-94-6173-318-4",

volume = "2",

pages = "563--568",

editor = "Robert Schilt",

booktitle = "Residues of veterinary drugs in food",

note = "null ; Conference date: 14-05-2012 Through 16-05-2012",

}

Reybroeck, W & Ooghe, S 2012, Validation of the microbiological inhibitor test Charm Blue Yellow II for screening for antimicrobials in milk. in R Schilt (ed.), Residues of veterinary drugs in food: Proceedings of the EuroResidue VII Conference, Egmond aan Zee, The Netherlands, 14-16 May 2012. vol. 2, pp. 563-568, EuroResidue VII Conference on Residues of Veterinary Drugs in Food, Egmond aan Zee, Netherlands, 14/05/12.

Validation of the microbiological inhibitor test Charm Blue Yellow II for screening for antimicrobials in milk. / Reybroeck, Wim ; Ooghe, Sigrid.

Residues of veterinary drugs in food: Proceedings of the EuroResidue VII Conference, Egmond aan Zee, The Netherlands, 14-16 May 2012. ed. / Robert Schilt. Vol. 2 2012. p. 563-568.

Research output: Chapter in Book/Report/Conference proceeding › C1: Articles in proceedings

TY - CHAP

T1 - Validation of the microbiological inhibitor test Charm Blue Yellow II for screening for antimicrobials in milk

AU - Reybroeck, Wim

AU - Ooghe, Sigrid

PY - 2012

Y1 - 2012

N2 - The Charm Blue Yellow II (Charm Sciences Inc., Lawrence, MA) is a broad-spectrum microbiological inhibitor test for the detection of antimicrobials in milk. The test was validated at ILVO-T&V according to Commission Decision 2002/657/EC. All ß-lactam antibiotics were detected at their respective MRL, except for desfuroylceftiofur. Moreover, all tetracyclines, 3 out of 5 sulfonamides and also tylosin, tilmicosin, gentamycin, neomycin, lincomycin and pirlimycin were detected at their respective MRL. The repeatability in scores for the Charm Blue Yellow II is not always as good as the repeatability in colour factors obtained for other similar microbiological tests. Interferences (false positive results) were observed for high somatic cell milk, for milk with a high fat content and for milk with a low or high protein content. These results are consistent with other inhibitory tests evaluated, with the exception of CMT (Copan Milk Test).

AB - The Charm Blue Yellow II (Charm Sciences Inc., Lawrence, MA) is a broad-spectrum microbiological inhibitor test for the detection of antimicrobials in milk. The test was validated at ILVO-T&V according to Commission Decision 2002/657/EC. All ß-lactam antibiotics were detected at their respective MRL, except for desfuroylceftiofur. Moreover, all tetracyclines, 3 out of 5 sulfonamides and also tylosin, tilmicosin, gentamycin, neomycin, lincomycin and pirlimycin were detected at their respective MRL. The repeatability in scores for the Charm Blue Yellow II is not always as good as the repeatability in colour factors obtained for other similar microbiological tests. Interferences (false positive results) were observed for high somatic cell milk, for milk with a high fat content and for milk with a low or high protein content. These results are consistent with other inhibitory tests evaluated, with the exception of CMT (Copan Milk Test).

M3 - C1: Articles in proceedings

SN - 978-94-6173-318-4

VL - 2

SP - 563

EP - 568

BT - Residues of veterinary drugs in food

A2 - Schilt, Robert

Y2 - 14 May 2012 through 16 May 2012

ER -

Validation of the microbiological inhibitor test Charm Blue Yellow II for screening for antimicrobials in milk

Abstract

Projects

VALIDATIEANTIBIO: Validation of screening tests of antibiotic residues for kit manufacturers

DETTECH: Development and validation of new methods for detection of residues

Cite this