Validation of the microbiological inhibitor test Charm Blue Yellow II for screening for antimicrobials in milk

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    The Charm Blue Yellow II (Charm Sciences Inc., Lawrence, MA) is a broad-spectrum microbiological inhibitor test for the detection of antimicrobials in milk. The test was validated at ILVO-T&V according to Commission Decision 2002/657/EC.
    All ß-lactam antibiotics were detected at their respective MRL, except for desfuroylceftiofur. Moreover, all tetracyclines, 3 out of 5 sulfonamides and also tylosin, tilmicosin, gentamycin, neomycin, lincomycin and pirlimycin were detected at their respective MRL.
    The repeatability in scores for the Charm Blue Yellow II is not always as good as the repeatability in colour factors obtained for other similar microbiological tests.
    Interferences (false positive results) were observed for high somatic cell milk, for milk with a high fat content and for milk with a low or high protein content. These results are consistent with other inhibitory tests evaluated, with the exception of CMT (Copan Milk Test).
    Original languageEnglish
    Title of host publicationResidues of veterinary drugs in food : Proceedings of the EuroResidue VII Conference, Egmond aan Zee, The Netherlands, 14-16 May 2012
    EditorsRobert Schilt
    Number of pages6
    Publication date2012
    ISBN (Print)978-94-6173-318-4
    Publication statusPublished - 2012
    EventEuroResidue VII Conference on Residues of Veterinary Drugs in Food - Egmond aan Zee, Egmond aan Zee, Netherlands
    Duration: 14-May-201216-May-2012

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