TY - JOUR
T1 - A comparative study of molecular and morphological methods of describing relationships between perennial ryegrass (Lolium perenne L.) varieties
AU - Roldan-Ruiz, Isabel
AU - van Eeuwijk, FA
AU - Gilliland, TJ
AU - Dubreuil, P
AU - Dillmann, C
AU - Lallemand, J
AU - De Loose, M
AU - Baril, CP
PY - 2001
Y1 - 2001
N2 - A sample set of registered perennial ryegrass varieties was used to compare how morphological characterisation and AFLP(R)(1) and STS molecular markers described variety relationships. All the varieties were confirmed as morphologically distinct, and both the STS and AFLP markers exposed sufficient genetic diversity to differentiate these registered ryegrass varieties. Distances obtained by each of the approaches were compared, with special attention given to the coincidences and divergences between the methods. When correlations between morphological, AFLP and STS distances were calculated and the corresponding scatter-plots constructed, the variety relationships appeared to be rather inconsistent across the methods, especially between morphology and the molecular markers. However, some consistencies were found for closely related material. An implication could be that these molecular-marker techniques, while not yet suited to certain operations in the traditional registration of new varieties, could be suitable methods for investigating disputable distinctness situations or possible EDV2 relationships, subject to establishing standardised protocols and statistical techniques. Some suggestions for such a protocol, including a statistical test for distinctness, are given.
AB - A sample set of registered perennial ryegrass varieties was used to compare how morphological characterisation and AFLP(R)(1) and STS molecular markers described variety relationships. All the varieties were confirmed as morphologically distinct, and both the STS and AFLP markers exposed sufficient genetic diversity to differentiate these registered ryegrass varieties. Distances obtained by each of the approaches were compared, with special attention given to the coincidences and divergences between the methods. When correlations between morphological, AFLP and STS distances were calculated and the corresponding scatter-plots constructed, the variety relationships appeared to be rather inconsistent across the methods, especially between morphology and the molecular markers. However, some consistencies were found for closely related material. An implication could be that these molecular-marker techniques, while not yet suited to certain operations in the traditional registration of new varieties, could be suitable methods for investigating disputable distinctness situations or possible EDV2 relationships, subject to establishing standardised protocols and statistical techniques. Some suggestions for such a protocol, including a statistical test for distinctness, are given.
U2 - 10.1007/s001220100571
DO - 10.1007/s001220100571
M3 - A1: Web of Science-article
VL - 103
SP - 1138
EP - 1150
JO - Theoretical and Applied Genetics
JF - Theoretical and Applied Genetics
SN - 0040-5752
IS - 8
ER -