A limited role for SsrA/B in persistent Salmonella Typhimurium infections in pigs

Filip Boyen, Frank Pasmans, Filip Van Immerseel, Eirwen Morgan, Nadine Botteldoorn, Marc Heyndrickx, Jiri Volf, Herman Favoreel, Jean-Pierre Hernalsteens, Richard Ducatelle, Freddy Haesebrouck

    Onderzoeksoutput: Bijdrage aan tijdschriftA1: Web of Science-artikelpeer review


    Virulence genes regulated by the SsrA/B system are indispensable for systemic disease in BALB/c mice. The role of this regulating system in the pathogenesis of Salmonella Typhimurium infections in pigs is not documented. In the present study, the interactions of Salmonella Typhimurium and an ssrA/B mutant were compared in vitro and in vivo. The ssrA/B mutant strain displayed decreased Salmonella Pathogenicity Island 2 (SPI-2) expression levels, showed a replication defect in mouse macrophages and was attenuated in a mouse model after oral inoculation. Using real time qRT-PCR and a porcine ileal loop model, it was shown that the ssrA/B mutant strain was not significantly attenuated in overall virulence and SPI-1 expression in specific. Flowcytometric analysis demonstrated that the ssrA/B mutant strain was defective in intracellular replication in porcine macrophages. After oral inoculation of piglets with the wild type strain or the ssrA/B mutant strain, the animals of both groups excreted Salmonella and were colonized by Salmonella to the same extent. In an intravenous mixed infection model, the ssrA/B mutant strain was defective in the colonization of several internal organs. These results suggest that the ssrA/B gene of Salmonella Typhimurium plays a limited role in the persistent intestinal colonization of pigs.
    Oorspronkelijke taalEngels
    TijdschriftVeterinary Microbiology
    Pagina's (van-tot)364-73
    Aantal pagina’s10
    PublicatiestatusGepubliceerd - 2008


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