In vitro inoculation methods are an efficient tool for the accurate study of the interaction between plants and parasitic nematodes. Obtaining disinfected nematodes is a crucial step in most of these methods. Most disinfection protocols involve surface sterilization of hatched juveniles, a procedure that usually leads to high mortality. This paper describes a new protocol for fast, inexpensive and easy disinfection of nematodes of the genus Globodera, directly from cysts. Mortality is low and varies between 10 and 40 % at the most. The surface-sterilized juveniles successfully infected in vitro-grown potato roots and reproduced on them.
|Tijdschrift||Fundamental And Applied Nematology|
|Publicatiestatus||Gepubliceerd - 1996|