A method to obtain disinfected Globodera infective juveniles directly from cysts

In vitro inoculation methods are an efficient tool for the accurate study of the interaction between plants and parasitic nematodes. Obtaining disinfected nematodes is a crucial step in most of these methods. Most disinfection protocols involve surface sterilization of hatched juveniles, a procedure that usually leads to high mortality. This paper describes a new protocol for fast, inexpensive and easy disinfection of nematodes of the genus Globodera, directly from cysts. Mortality is low and varies between 10 and 40 % at the most. The surface-sterilized juveniles successfully infected in vitro-grown potato roots and reproduced on them.