Aberrant genome size and instability of Phytophthora ramorum oospore progenies

Annelies Vercauteren, Xavier Boutet, Liesbet D'hondt, Erik Van Bockstaele, Martine Maes, Leen Leus, Anne Chandelier, Kurt Heungens

Onderzoeksoutput: Bijdrage aan tijdschriftA1: Web of Science-artikelpeer review


The functionality of the sexual cycle in the heterothallic pathogen Phytophthora ramorum, causal agent of Sudden Oak Death, has recently been demonstrated. Sexual reproduction could create genotypic variation and increase the pathogen' s ability to adapt to other host plants or changing environments. Genetic characterization using co-dominant microsatellite markers and flow cytometry of single-oospore progeny of crosses between a European A1 isolate and North American or European A2 isolates revealed a considerable number of non-Mendelian inheritance events. This includes inheritance of more than two alleles at a locus and non-inheritance of alleles from one parent at another locus. The progenies were mitotically unstable: zoospore and hyphal tip derivatives of the progenies showed genotypic rearrangements and phenotypic variation. Flow cytometry confirmed variation and instability in DNA content of the single-oospore progenies. This indicates that single-oospore progenies not only display aberrant genomic and phenotypic variation due to meiotic irregularities, but also extra variation as a result of post-meiotic genomic rearrangements.
Oorspronkelijke taalEngels
TijdschriftFungal Genetics and Biology
Pagina's (van-tot)537-543
Aantal pagina’s7
PublicatiestatusGepubliceerd - 2011


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