An imaging study and spectroscopic curing analysis on polymers for synthetic whole-cell receptors for bacterial detection

Stella Givanoudi, Alessia Gennaro, Derick Yongabi, Peter Cornelis, Gideon Wackers, Alberto Lavatelli, Johan Robbens, Marc Heyndrickx, Michael Wübbenhorst, Patrick Wagner

Onderzoeksoutput: Bijdrage aan tijdschriftA1: Web of Science-artikel


Surface imprinted polymers (SIPs) are versatile receptors in bioanalytical applications for the selective detection of cells and microorganisms such as bacteria. One of the synthesis routes is the so-called stamping method in which template bacteria are pressed mechanically into a thin, gel-like polyurethane layer, which is then cured in the presence of the templates to create cell-specific binding pockets on the polymer. The present work focusses on two specific steps of the imprinting protocol: first, we evaluate the sedimentation of two different groups of bacteria, Escherichia coli and Escherichia blattae, on silicone stamps with respect to the resulting surface coverage, which is a key factor for the efficiency of the imprinting process. Second, we analyse the temperature dependence of the thermal- and dielectric properties of polyurethane during curing by dielectric- and pyroelectric spectroscopy. This provides information for improved curing protocols and on the stability of SIP materials at elevated temperatures.
Artikel nummer SD0802
TijdschriftJapanese Journal of Applied Physics
StatusGepubliceerd - 2020

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