Analysis of clones carrying repeated DNA-sequences in 2YAC libraries of Arabidopsis-thaliana DNA

R Schmidt; J Putterill; J West; Gerda Cnops; F Robson; G Coupland; C Dean

doi:10.1111/j.1365-313X.1994.00735.x

Analysis of clones carrying repeated DNA-sequences in 2YAC libraries of Arabidopsis-thaliana DNA

R Schmidt
, J Putterill
, J West
, Gerda Cnops
, F Robson
, G Coupland
, C Dean

Onderzoeksoutput: Bijdrage aan tijdschrift › A1: Web of Science-artikel › peer review

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YAC clones carrying repeated DNA sequences from the Arabidopsis thaliana genome have been characterized in two widely used Arabidopsis YAC libraries, the EG library and the EW library. Ribosomal, chloroplast and the paracentromeric repeat sequences are differentially represented in the two libraries. The coordinates of YAC clones hybridizing to these sequences are given. A high proportion of EG YAC clones were classified as containing chimaeric inserts because individual clones carried unique sequences and repetitive sequences originating from different locations in the genome. None of the EW YAC clones analysed were chimaeric in this way. YAC clones carrying tandemly repeated sequences, such as the paracentromeric or rDNA sequences, exhibited a high degree of instability. These observations need to be taken into account when using these libraries in the development of a physical map of the Arabidopsis genome and in chromosome walking experiments.

Oorspronkelijke taal	Engels
Tijdschrift	Plant Biotechnology Journal
Volume	5
Exemplaarnummer	5
Pagina's (van-tot)	735-744
Aantal pagina’s	10
DOI's	https://doi.org/10.1111/j.1365-313X.1994.00735.x
Publicatiestatus	Gepubliceerd - 1994

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10.1111/j.1365-313X.1994.00735.x

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@article{8caede04844849bfbcc142e098b2f5e9,

title = "Analysis of clones carrying repeated DNA-sequences in 2YAC libraries of Arabidopsis-thaliana DNA",

abstract = "YAC clones carrying repeated DNA sequences from the Arabidopsis thaliana genome have been characterized in two widely used Arabidopsis YAC libraries, the EG library and the EW library. Ribosomal, chloroplast and the paracentromeric repeat sequences are differentially represented in the two libraries. The coordinates of YAC clones hybridizing to these sequences are given. A high proportion of EG YAC clones were classified as containing chimaeric inserts because individual clones carried unique sequences and repetitive sequences originating from different locations in the genome. None of the EW YAC clones analysed were chimaeric in this way. YAC clones carrying tandemly repeated sequences, such as the paracentromeric or rDNA sequences, exhibited a high degree of instability. These observations need to be taken into account when using these libraries in the development of a physical map of the Arabidopsis genome and in chromosome walking experiments.",

author = "R Schmidt and J Putterill and J West and Gerda Cnops and F Robson and G Coupland and C Dean",

year = "1994",

doi = "10.1111/j.1365-313X.1994.00735.x",

language = "English",

volume = "5",

pages = "735--744",

journal = "Plant Biotechnology Journal",

issn = "1467-7652",

publisher = "Wiley-Blackwell Publishing Ltd",

number = "5",

}

TY - JOUR

T1 - Analysis of clones carrying repeated DNA-sequences in 2YAC libraries of Arabidopsis-thaliana DNA

AU - Schmidt, R

AU - Putterill, J

AU - West, J

AU - Cnops, Gerda

AU - Robson, F

AU - Coupland, G

AU - Dean, C

PY - 1994

Y1 - 1994

N2 - YAC clones carrying repeated DNA sequences from the Arabidopsis thaliana genome have been characterized in two widely used Arabidopsis YAC libraries, the EG library and the EW library. Ribosomal, chloroplast and the paracentromeric repeat sequences are differentially represented in the two libraries. The coordinates of YAC clones hybridizing to these sequences are given. A high proportion of EG YAC clones were classified as containing chimaeric inserts because individual clones carried unique sequences and repetitive sequences originating from different locations in the genome. None of the EW YAC clones analysed were chimaeric in this way. YAC clones carrying tandemly repeated sequences, such as the paracentromeric or rDNA sequences, exhibited a high degree of instability. These observations need to be taken into account when using these libraries in the development of a physical map of the Arabidopsis genome and in chromosome walking experiments.

AB - YAC clones carrying repeated DNA sequences from the Arabidopsis thaliana genome have been characterized in two widely used Arabidopsis YAC libraries, the EG library and the EW library. Ribosomal, chloroplast and the paracentromeric repeat sequences are differentially represented in the two libraries. The coordinates of YAC clones hybridizing to these sequences are given. A high proportion of EG YAC clones were classified as containing chimaeric inserts because individual clones carried unique sequences and repetitive sequences originating from different locations in the genome. None of the EW YAC clones analysed were chimaeric in this way. YAC clones carrying tandemly repeated sequences, such as the paracentromeric or rDNA sequences, exhibited a high degree of instability. These observations need to be taken into account when using these libraries in the development of a physical map of the Arabidopsis genome and in chromosome walking experiments.

U2 - 10.1111/j.1365-313X.1994.00735.x

DO - 10.1111/j.1365-313X.1994.00735.x

M3 - A1: Web of Science-article

SN - 1467-7652

VL - 5

SP - 735

EP - 744

JO - Plant Biotechnology Journal

JF - Plant Biotechnology Journal

IS - 5

ER -

Analysis of clones carrying repeated DNA-sequences in 2YAC libraries of Arabidopsis-thaliana DNA

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