TY - JOUR
T1 - Analysis of EU priority polycyclic aromatic hydrocarbons in food supplements using high performance liquid chromatography coupled to an ultraviolet, diode array or fluorescence detector
AU - Danyi, Sophie
AU - Brose, François
AU - Brasseur, Catherine
AU - Schneider, Yves-Jacques
AU - Larondelle, Yvan
AU - Pussemier, Luc
AU - Robbens, Johan
AU - De Saeger, Sarah
AU - Maghuin-Rogister, Guy
AU - Scippo, Marie-Louise
PY - 2009
Y1 - 2009
N2 - High performance liquid chromatography coupled to an ultraviolet, diode array or fluorescence detector (HPLC/UV-FLD) has been used to set up a method to detect the 15(+1) EU priority polycyclic aromatic hydrocarbons (PAHs) in food supplements covering the categories of dried plants and plant extracts excluding oily products. A mini validation was performed and the following parameters have been determined: limit of detection, limit of quantification, precision, recovery and linearity. They were in close agreement with quality criteria described in the Commission Regulation (EC) No 333/2007 concerning the PAH benzo[a]pyrene in foodstuffs, except the not fluorescent cyclopenta[c,d]pyrene for which the UV detection leads to a higher limit of detection. Analysis of twenty commercial food supplements covering mainly the class of dried plants was performed to evaluate their PAHs contamination levels and to test the applicability of the method to various plant matrices. Fifty percent of analyzed samples showed concentration exceeding 2 microgkg(-1) for one or more PAHs.
AB - High performance liquid chromatography coupled to an ultraviolet, diode array or fluorescence detector (HPLC/UV-FLD) has been used to set up a method to detect the 15(+1) EU priority polycyclic aromatic hydrocarbons (PAHs) in food supplements covering the categories of dried plants and plant extracts excluding oily products. A mini validation was performed and the following parameters have been determined: limit of detection, limit of quantification, precision, recovery and linearity. They were in close agreement with quality criteria described in the Commission Regulation (EC) No 333/2007 concerning the PAH benzo[a]pyrene in foodstuffs, except the not fluorescent cyclopenta[c,d]pyrene for which the UV detection leads to a higher limit of detection. Analysis of twenty commercial food supplements covering mainly the class of dried plants was performed to evaluate their PAHs contamination levels and to test the applicability of the method to various plant matrices. Fifty percent of analyzed samples showed concentration exceeding 2 microgkg(-1) for one or more PAHs.
KW - Chemical Fractionation
KW - Chromatography, High Pressure Liquid
KW - Dietary Supplements
KW - European Union
KW - Polycyclic Hydrocarbons, Aromatic
KW - Reproducibility of Results
KW - Solvents
KW - Spectrometry, Fluorescence
KW - Spectrophotometry, Ultraviolet
U2 - 10.1016/j.aca.2008.11.049
DO - 10.1016/j.aca.2008.11.049
M3 - A1: Web of Science-article
C2 - 19166736
VL - 633
SP - 293
EP - 299
JO - Analytica Chimica Acta
JF - Analytica Chimica Acta
SN - 0003-2670
IS - 2
ER -